X-盒结合蛋白1、血管内皮生长因子C在肝癌中的表达及意义

The expression and significance of the correlation factor of unfolded protein response： X-box bind- ing protein 1, vascular endothelial growth factor C in hepatoma

目的检测X-盒结合蛋白1（XBPl），血管内皮生长因子c（VEGF—C）在肝癌组织中的表达，探讨在肝癌发生发展过程中存在未折叠蛋白反应（UPR）的激活。方法采用逆转录一聚合酶链反应（RT—PCR）法检测术中取的新鲜42例肝癌标本、15例同个体癌旁1．0em肝组织和15例正常肝组织中XBPl、VEGF—C的mRNA表达，应用Westernblot法检测57例肝癌组织和正常肝组织标本中XBPl、VEGF—C在蛋白水平的表达。结果肝癌组织、癌旁肝组织、正常肝组织中XBPImRNA相对表达量分别为0．4396±0．0241、0．4152±0．0252、0．4095±0．0149，XBPlmRNA在3组之间的表达呈下降趋势（P〈0．05）；肝癌组织、癌旁肝组织、正常肝组织中VEGF—CmRNA相对表达量分别为0．4447±0．0335、0．4195±0．0334、0．4019±0．0259，VEGF—CmRNA在3组之间的表达呈下降趋势（P〈0．05）。在肝癌组织和正常肝组织中均有两种蛋白的表达，但肝癌组织中的表达明显高于正常肝组织。结论肝癌组织中XBPI、VEGF—CmRNA和蛋白的表达一致，均为高表达。

Objective To detect the express of X-box binding protein 1 （ XBP1 ）, vascular endo- thelial growth factor C （ VEGF-C ）, mRNA in the tissue of hepatoma; demonstrating that it exist activation of unfold protein response （UPR） in process of growth, and the expression of UPR was higher than normal tissue ; approaching the mechanism of action in the growth of hepatoma, searching the new target for the therapy of hepatoma. Methods To study the mRNA of XBP1, VEGF-C in 42 case example of hepatome, 15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by reverse tran- scription-polymerase chain reaction （RT-PCR）. To qualitation check the expression of XBP1 and VEGF-C in the level of protein in 57 case example of hepatome and normal hepatic tissues, by Western blotting. Re- suits The express of XBP1 mRNA in hepatoma tissue, adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was0. 4396 ± 0. 0241, 0. 4152± 0. 0252, 0. 4095 ± 0. 0149 ; The express of XBP1 mI：INA was higher in hepatoma than others （P 〈 0. 05 ）. The express of VEGF-C mRNA in hepatoma tissue, adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4447 ± 0. 0335, 0. 4195 ± 0. 0334, 0. 4019 ± 0. 0259. The express of VEGF-C mRNA was higher in hepatoma than others （ P 〈 0. 05 ）. The experiment indicate ： the two protein all express in the hepatoma tissue and normal hepat- ic tissue, but they were higher in hepatoma tissue than in the normal hepatic tissue. Conclusion It exist activation of UPR in process of growth. The express of XBP1, VEGF-C protern and their mRNA was ac- cordant, all of them were higher in hepatoma tissue than in normal hepatic tissue. The changes of these gene and protern is possible to have the relationship with the carcinogenesis, growth and transfer of hepato- ma. And it may have significant effect in molecule mechanism of hepatoma. It also descripte that unfold protein response may have very important role in the pocess of carcinogenesis.