miR-155和miR-30a在胃癌组织中的表达及临床意义

Expression of miR-155 and miR-30a in gastric carcinoma and the clinical significance

目的探讨胃癌组织和癌旁组织miR-155和miR-30a的表达差异及其临床意义。方法收集手术切除的胃癌标本52例，采用实时荧光定量逆转录一聚合酶链反应（RT—qPCR）检测癌及癌旁组织标本miR-155和miR-30a的表达水平，分析其与临床病理的关系。结果miR-155在胃癌组织中表达量（0．844-2．27）显著高于其在配对癌旁组织中的表达值（0．28±0．56，P〈0．05）；miR-155高表达与淋巴结转移有关（P〈0．05）。miR-30a在胃癌组织中表达量（3．16±8．11）显著低于其在配对癌旁组织中的表达值（15．87±35．58，P〈0．05）。miR-30a的低表达与肿瘤侵犯层次、淋巴结转移有关（P〈0．05）。结论miR-155和miR-30a与胃癌发生发展密切相关，可能成为胃癌的潜在诊断及治疗靶点。

Objective： To investigate the expression of miR-155 and miR-30a in gastric carcinoma and the clinical significance. Methods Fifty-two pairs of human gastric carcinoma tissue samples were ob- tained from patients who underwent surgical resection. The level of miR-155 and miR-30a in 52 gastric car- cinoma and the adjacent normal tissues was quantified by real-time fluorescent quantitative reverse tran- scriptase-polymerase chain reaction （RT-qPCR）, and the relationship between miR-155 and miR-30a level with clinicopathological factors was explored. Results The expression of miR-155 in gastric carcinoma tis- sues （0. 84 ± 2. 27 ） was significantly increased as compared with that in the adjacent normal tissues （0. 28 ± 0. 56 ,P 〈 0.05 ）. The up-regulated expression of miR-155 was associated with lymph node metas- tases in gastric carcinoma （P 〈 0.05 ）. The expression of miR-30a in gastric cancer tissues （3.16 ±8. 11 ） was significantly lower than that in the adjacent normal tissues （ 15.87 ± 35.58, P 〈 0. 05 ）. The low expression of miR-30a was associated with lymph node metastases and local invasion in gastric carcinoma （ P 〈 0. 05 ）. Conclusion miR-155 and miR-30a may play important roles in the development and progres- sion of gastric carcinoma, and they may be potential biomarkers in the diagnosis and treatment of gastric carcinoma.