摘要
目的观察酸感受离子通道（ASICs）激活／抑制对氯化锂一匹罗卡品大鼠的癫痫皮层脑电图癫痫放电的影响。方法制作匹罗卡品大鼠癫痫模型，分别腹腔注射磷酸盐缓冲液（PBS）、阿米洛利、左乙拉西坦或酸性液体，记录脑电图癫痫样发电次数、波幅、放电间期变化。结果在注射后60～90min，与正常对照组比较，ASICs抑制组癫痫样放电次数（0．69±0．08）、波幅（0．70±0．16）均明显下降（P〈0．05），放电间期（1．46±0．18）延长（P〈0．05）；ASICs激活组在注射后30min内，癫痫样放电次数增高（1．05±0．07）（P〈0．05）。结论阿米洛利能抑制锂一匹罗卡品诱导的癫痫发作，酸性液体可促进癫痫发作，其作用机制可能与抑制或激活ASICs通道有关。
Objective To study the effects of acid-sensor ion channels activation or inhibition on the epileptic discharge in EEG of epilepsy rat model induced by Lithium-pilocarpine. Methods Seizures were intraperitoneally induced in rats with lithium-pilocarpine. The rats were divided into four groups： the placebo group, the ASICs inhibitor group, the positive control group and the ASICs activator group by ad- ministrating intraperitoneally PBS, amiloride, Levetiracetam or acidic liquids, respectively. The number of discharge, and amplitude and interval of discharge were selected as the indicators for comparison. Results Sixty-ninety min after the injection, the number of discharge （0. 69 ± 0.08 ） and the amplitude （0. 70± 0. 16） were reduced in the ASICs inhibitor group, while the interval of discharge was increased （1.46 ± 0. 18 ） （ P 〈 0. 05 ） as compared with the placebo group. In the ASICs activator group, the number of discharge （ 1.05 ±0. 07） was increased 30 min after injection. Conclusion Amiloride can inhibit epilepsy induced by lithium-pilocarpine, while acid liquids exert the opposite effects, which may be concerned with the inhibition or activation of ASICs channel.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第8期1566-1568,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30900459）
武汉大学青年教师资助项目（3082012）
国家教育部新教师基金资助项目（090392）
湖北省教育厅科学技术研究计划指导性项目（B类）（B20090102）
