HPLC法测定温毒清浸膏中栀子苷的含量

Content determination of geniposide in Wenduqing extractum by HPLC

采用HPLC方法测定温毒清浸膏中栀子苷的含量。流动相:乙腈-水(15∶85);流速:1 mL/min;检测波长:238 nm;色谱柱:Diamonsi L C18(5μm,200 mm×4.6 mm);柱温:25℃;理论塔板数不低于1 500。结果显示,栀子苷进样量在0.060-0.600μg范围内有良好的线性关系,平均回收率99.32%,RSD为1.26%。表明该方法准确可靠,重现性好,可作为温毒清浸膏的质量控制方法。

Objective： To determine the content of geniposide in Wenduqing extractum by HPLC. Methods： The mobile phase was acetonitrile and water （15：85）with the flow rate of 1 mL/min at 238 nm, the chromatographic column was Diamonsi L Cts （5 μm,200 min×4.6 mm） at 25℃, and theoretical plate number was no less than 1 500. Results： There was a good linearity for geniposide in Wenduqing extractum in the range of 0.060-0.600μg with average recovery rate of 99.32% and RSD of 1.26%. Conclusion： HPLC was accuracy and repeatability. suitable to use to control the quality of Wenduqing extractum for its accuracy and repeatability,