温阳利水法结合负载自体抗原DC-CIK细胞治疗恶性腹水30例

Clinical Study on 30 Cases of Malignant ascites Treated by Yang-warming and water retention-alleviating Method and Immune Cell of DC-CIK Treatment

目的:探讨温阳利水中药联合DC-CIK免疫细胞治疗恶性腹腔积液的控制情况及对腹水细胞凋亡的影响。方法:90例恶性腹水患者随机分为3组,腹腔化疗组(采用腹腔灌注化疗)30例,免疫细胞组(采用DC-CIK细胞治疗)30例,中药细胞组(免疫细胞组基础上结合温阳利水中药)30例,对比治疗前及治疗后腹水细胞凋亡水平(APO),腹水控制有效率及KPS评分情况。结果:APO水平方面,中药细胞组在治疗前后变化有统计学意义,与腹腔化疗组、免疫细胞组比较无统计学意义(P>0.05);腹水控制有效率方面,中药细胞组同腹腔化疗组比较有统计学意义(P<0.05),同免疫细胞组比较无统计学意义(P>0.05)。KPS评分方面,中药细胞组同腹腔化疗组及免疫细胞组比较均有统计学意义(P<0.05)。结论:温阳利水法结合负载自体抗原DC-CIK免疫细胞治疗,能有效促进恶性腹水细胞凋亡,提高腹水控制的有效率,较好的改善患者生存质量。

Objective：To observe the effect of method of warming Yang and alleviating water retention and immune cell treatment of DC-CIK.Methods：90 cases were randomized into 3 groups：intraperitoneal chemotherapy group（30 case）,DC-CIK immune cell group（30 case）,immune cell treatment combined with Yang-warming-and-water-retention-alleviating method group（30 case）.The changes of apoptosis（APO）in ascites,the effective rate of control ascites and score of KPS were observed and compared.Results：Before and after treatment,the level of APO in combined treatment group was statistically significant,but compared with intraperitoneal chemotherapy and the immune cells groups it had no significant difference（P0.05）.For efficient control of ascites,it was significantly different between combined treatment group and intraperitoneal chemotherapy group（P0.05）,it had no significant difference between combined treatment group and immune cell group（P0.05）.For score of KPS：combined treatment group was statistically significant compared with intraperitoneal chemotherapy group or immune cell group（P0.05）.Conclusion：The method of warming Yang and alleviating water retention and immune cell treatment of DC-CIK could promote apoptosis of malignant ascites,increase the control efficiency,and further improve the quality of life of patients