清肠化湿方对TNBS诱导大鼠UC模型紧密连接蛋白的影响

Effects of Qingchang Huashig Decoction on Tight Junction Proteins in TNBS-induced Ulcerative Colitis Model Rats

目的:观察三硝基苯磺酸(TNBS)法诱导大鼠溃疡性结肠炎模型结肠黏膜occludin、claudin-1蛋白的变化特点及清肠化湿方对occludin、claudin-1蛋白表达的影响。方法:采用TNBS诱导大鼠溃疡性结肠炎模型,并随机分为正常对照组、模型对照组、清肠化湿方组、SASP组,治疗10天后处死大鼠取新鲜结肠标本,观察黏膜大体形态及组织学改变,并采用Western blot法检测occludin、claudin-1蛋白表达水平。结果:模型对照组occludin、claudin-1蛋白表达均明显低于正常对照组,清肠化湿方组occludin、claudin-1的表达高于模型对照组,同时,清肠化湿方组大鼠结肠形态及组织学损伤评分均有降低。结论:清肠化湿方对UC模型大鼠具有治疗作用,增强occludin、claudin-1的表达,恢复正常的肠粘膜屏障功能可能是其作用机制之一。

Objective：To observe the effect of Qingchang Huashig Decoction（QD）on the protein expressions and change character of occludin and claudin-1 in colonic mucosa of rats with ulcerative colitis（UC）.Methods：Rat UC model induced by trinitrobenzene-sulfonic acid（TNBS）were randomly divided into normal group,model group,QD group and SASP group.The rats were sacrificed to get their colonic mucosa and extract the whole-cell protein after 10d of treatment.Western blot was performed to detect the protein levels of occludin and claudin-1.Results：The relative protein expressions of occludin and claudin-1 in the model group were all lower than those in the normal group significantly.The relative protein expressions of occludin and claudin-1 in the QD group were higher than that in the model group.The colonic configurative and histological injury score were declined.Conclusion：Qingchang Huashig Decoction can treat ulcerative colitis.One of the mechanisms may be that Qingchang Huashig Decoction can increase the relative protein expressions of occludin and claudin-1 and restore the normal intestinal mucosal barrier function.