局部应用菟丝子总多糖对家兔骨折修复中TGF-β1表达的实验研究

TGF-β1 Expression of Local Application of Total Polysaccharide from Cuscuta on Rabbits Fracture Healing

目的:研究局部运用菟丝子总多糖在促进家兔骨折修复中TGF-β1的表达及其机理。方法:选用健康成年家兔63只,体重2.5 kg左右。随机分为3组(即菟丝子组、BMP组和空白组),每组21只。在小腿中段前外侧逐层切开皮肤,分离组织,以线锯于胫骨中段截骨约0.5 cm,手法复位并维持。菟丝子组、BMP组分别在骨缺损断端间置入菟丝子总多糖凝胶、骨形成蛋白填充于缺损处;空白组断端间不作处理。三组均予缝合、夹板外固定。术后于2、4、8周分批处死实验动物7只,取标本行TGF-β1免疫组化的方法进行评估。结果:中药组、BMP组TGF-β1免疫组化积分光密度值与空白组比较,都有显著性差异(P<0.01或P<0.05);中药组与BMP组比较无统计学意义(P>0.05)。结论:菟丝子总多糖可促进TGF-β1参与调节骨折修复。一定程度证实了中药菟丝子对骨折损伤的修复作用。

Objective：To study TGF-β1 expression and the related mechanism about the effect of local application of the total polysaccharide from Cuscuta on accelerating fracture healing.Methods：63 healthy adult rabbits,weight about 2.5 kg,were randomly divided into three groups：the Cuscuta chinensis group,the bone morphogenetic protein（BMP）group,the control group,21 in every group.After skin incision layer by layer in anterolateral cross leg,separated tissue,osteotomy was performed in middle cross section of tibia with Fret saw,length is about 0.5cm,then treated with manual reduction and maintenance.The Cuscuta chinensis group and BMP group were filled with The total polysaccharide from Cuscuta and BMP respectively,no treatment was given to the control group.Furthermore,three group were treated with suture and splint external fixation.At 2 weeks,4 weeks,8 weeks after respective treatments,7 rabbits in each group were randomly selected and sacrificed.Tissue samples were collected and analyzed by TGF-β1 immunohistochemistry.Results：Compared with the control group,the value of TGF-β1 integral optical density in the Cuscuta chinensis group and BMP group showed significant difference,（P〈0.01 or P〈0.05）;while the difference was not statistically significant between the Cuscuta chinensis group and BMP group（P〉0.05）.Conclusion：By up-regulating the levels of TGF-β1,the total polysaccharide from Cuscuta have effectiveness on accelerating fracture healing.In some extent,this can be thought as the evidence of fracture reparing effect of Cuscuta chinensis.