鹿茸水提物模拟胃肠消化物对小鼠脾细胞增殖的影响

Effect of simulated gastrointestinal digest of aqueous extract of velvet antler on murine splenocytes proliferation

研究鹿茸水提取物模拟胃肠消化物(AEVA-SGD)的分离组分对小鼠脾淋巴细胞增殖的影响。AEVA-SGD的分离采用凝胶层析法和RP-HPLC法。应用WST-8法检测各分离组分对小鼠脾淋巴细胞增殖的影响,采用高分辨质谱对有效分离组分进行初步鉴定。结果显示,AEVA-SGD经Sephadex LH-20分离后得到6个组分(A～F)。其中,组分F对小鼠脾细胞增殖的影响最为显著,可单独促进小鼠脾细胞增殖,但对ConA诱导的T淋巴细胞增殖有抑制作用。经RP-HPLC将组分F分为3个部分,初步鉴定组分F1为鸟嘌呤,F2为1-甲基鸟嘌呤和黄嘌呤,F3为多种小肽的混合物。组分F3对小鼠脾细胞增殖的影响最大,组分F对小鼠脾细胞增殖的影响是其各分离组分协同作用的结果。

The aim of the present study was to investigate the effect of fractions from simulated gastrointestinal digest of aqueous extract of velvet antler（AEVA-SGD） on murine splenocytes proliferation. AEVA-SGD was fractionated by gel chromatography and RP-HPLC consecutively. The effect of each fraction on the proliferation of murine splenocytes was detected by WST-8. The effective fractions were preliminary identified by high resolution mass spectrometry. The results showed that AEVA-SGD was separated into six fractions（ANF） by Sephadex LH-20. Fraction F（Fr. F） stimulated the proliferation of resting murine splenocytes,whereas inhibited the proliferation of ConA-stimulated murine splenocytes. The effect of Fr. F on the proliferation of murine splenocytes was more effective than the other fractions. After separation by RP-HPLC,Fr. F was fractionated into three parts. The main components of each part were preliminary identified as=guanine （Fr.F1）, 1- methylguanine and xanthine（Fr. F2）,a mixture of small peptides（Fr. F3）. Compared with Fr. F1 and Fr. F2,the effect of Fr. F3 on the proliferation of murine splenocyte was more effective. The total effect of Fr, F on the proliferation of murine splenocyte was due to the synergistic effects of its fractions.