摘要
In this study, electrospray ionization mass spectrometry （ESI-MS） was used to investigate interaction of 21 flavonoids （10 aglycones and 11 glycosides） with the parallel quadruplex structure [d（TGGGGT）]4. Relative binding affinities of flavonoids toward [d（TGGGGT）]4 were estimated based on the fraction of bound DNA. It was found that [d（TGGGGT）]4 showed a binding preference to the flavonoid glycosides over flavonoid aglycones. It was d duced that glycosylation played a key role for the [d（TGGGGT）]a-binding properties of flavonoid glycosides. Upon collision-induced dissociation, complexes of flavonoid/[d（TGGGGT）]4 underwent the loss of flavonoids, suggesting an end-stacking binding mode. The current work demonstrates that ESI-MS is a powerful tool in the study of irheraction between drugs and nucleic acids.
In this study, electrospray ionization mass spectrometry （ESI-MS） was used to investigate interaction of 21 flavonoids （10 aglycones and 11 glycosides） with the parallel quadruplex structure [d（TGGGGT）]4. Relative binding affinities of flavonoids toward [d（TGGGGT）]4 were estimated based on the fraction of bound DNA. It was found that [d（TGGGGT）]4 showed a binding preference to the flavonoid glycosides over flavonoid aglycones. It was d duced that glycosylation played a key role for the [d（TGGGGT）]a-binding properties of flavonoid glycosides. Upon collision-induced dissociation, complexes of flavonoid/[d（TGGGGT）]4 underwent the loss of flavonoids, suggesting an end-stacking binding mode. The current work demonstrates that ESI-MS is a powerful tool in the study of irheraction between drugs and nucleic acids.
基金
Acknowledgement This work was supported by the National Natural Science Foundation of China （No. 28073137）.
