社区获得性耐甲氧西林金黄色葡萄球菌基因分型及耐药性研究

Drug resistance and gene typing of community-associated methicillin-resistant Staphylococcus aureus

目的了解社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)分离株的耐药性及遗传背景。方法对CA-MRSA进行多位点序列分型(MLST)检测,采用应用多重聚合酶链式反应(PCR)技术对CA-MRSA进行SCCmec基因分型;采用微量稀释法检测CA-MRSA抗菌药物敏感性。结果 76株社区获得性金黄色葡萄球菌共检测出4株CA-MRSA,分别为4个基因型,分别为ST88-MRSA-Ⅳa;ST121-MRSA-Ⅳa;ST221-MRSA-Ⅳa;ST82-MRSA-Ⅳa;CA-MRSA对非β-内酰胺类抗生素多为敏感。结论滨州医学院附属医院分离到4株CA-MRSA,表明已有CA-MRSA的散发流行,但没有发现与国际主要CA-MRSA流行株相同克隆株的发现,MLST联合SCCmec基因分型方法是CA-MRSA分子流行病学研究简便快速的方法。

Objective To investigate the drug resistance and genetic background of community- associated methicillin-resistant Staphylococcus aureus. Methods Multilocus Sequence Typing（MLST）technique were introduced to identify sequence types（ST） of CA-MRSA, SCCmec types were determined by Multiplex PCR methods. The susceptibility to antimicrobial agents was tested by micro-dilution method. MLST plus SCCmec typing of these strains were used to genotype them. The correlation and homology among the isolates were reflected by using the clustering analysis at the same time. Results Among the 76 isolates of community-associated Staphylococcus aureus, 4 isolates were belonged to CA-MRSA. Among 4 isolates, there were 4 different gene typing： ST88-MRSA- 1Va; ST121-MRSA-IVa; ST221-MRSA-IVa; ST82-MRSA-IVa; CA-MRSA strains tend to be susceptible to a wide variety of non-β-lactam antibiotics. Conclusions 4 isolates belonged to CA-MRSA were be separated in Binzhou Medical University Hospital, but found no with major global epidemic strains the same cloning strains of the find, MLST combined with SCCmec types is a rapid and simple CA-MRSA genotyping method.