“恩钿”月季组培技术研究

Study on Tissue Culture Technique of China Rose of 'Entian'

以"恩钿"月季茎段上不同部位、不同季节的芽为外植体,研究了"恩钿"月季组培苗的最佳培养基。结果表明:外植体选取时间以春季为佳,选取部位以枝条中、上部为好,适宜的增殖培养基为:MS+6-BA 1.0mg/L+NAA 0.1mg/L,最佳生根培养基为:1/2MS+NAA 0.2mg/L+300mg/L活性炭;练苗基质以蛭石为佳。

Taking the bud of stem-segment in different seasons and different positions as the explants, the tissue culture technique was studied. The results showed that the spring was the best time for selection of explants;the medium and top portion of trees were the right position for the explants cut. The proliferation media formula was MS＋6-BA 1.0 mg/L＋NAA 0. 1 mg/L; the media formula for rooting was 1/2MS＋NAA 0. 2 mg/L＋300 mg/L active carbon. Vermiculite was taken as the media for domestication.