洋桔梗组培苗生产关键技术研究

Study on Key Technology of Production Tissue Culture Seedling of Enstoma grandiflorun

以开粉色花的洋桔梗包衣种子为试材,通过叶片诱导、单芽增殖和生根等培养基的筛选,研究了洋桔梗组培苗生产关键技术。结果表明:叶片诱导的适合培养基为MS+6-BA 0.4mg/L+IBA 0.1mg/L,每叶盘分化的正常芽为5个左右;单芽诱导的适合培养基为MS+6-BA0.2mg/L+IBA 0.05mg/L,每母株分化的正常芽为5个左右,并有55%的芽达生根标准;生根的适合培养基为1/2MS+IBA 1.0mg/L,生根率达95%。

Taking the capsuled of Enstoma grandi：florun with pink flower as the material, the key technologies of production tissue culture seedling of Enstoma grandiflorun were studied. The mediums of leaves induction,single bubs enrichment culture and rooting were elected and the other methods of production were summarized. The results showedthat the best medium of leaves induction was MS＋6-BA 0. 4 mg/L＋IBA 0. 1 mg/L and the normal bubs every leaf was 5 in it. The best medium of single hubs enrichment was MS＋6-BA 0. 2 mg/L＋IBA 0. 05 mg/L and the normal bubs every single bubs was 5 in it. The best medium of rooting was 1/2MS＋IBA 1.0 mg/L and rooting rate was 95% in it.