摘要
目的建立长期慢性不可预知性应激刺激大鼠动物模型,观察大鼠应激后全身状态及行为学的改变。方法雄性Wistar大鼠32只,12周龄,分为实验组和对照组,再随机分为3周及6周时间组,各为8只大鼠。实验组进行随机应激刺激,建立慢性不可预知性应激动物模型,对照组不加任何刺激,正常饲养。各组大鼠每周测量体质量,观察动物变化,于实验各时间点进行旷场行为学测试,记录水平活动及垂直活动得分,进行比较。结果实验组大鼠体质量增加明显慢于对照组,行为学测试各实验组与对照组均有显著性差异(P<0.05)。结论大鼠的行为学活动在长期慢性不可预知性应激刺激后明显改变,体质量增长速率减缓,表明大鼠在长期应激刺激下正常生长、生活会造成很大的障碍。
Objective To observe the changes of general body state of rats in stress state induced by chronic unpredictable stress experiments. Methods Thirty two 12-week-old male Wistar rats were randomly divided into 2 groups, the control group and the experimental group. Each group had eight rats, was divided into two subgroups according to execution time （3-week subgroup and 6-week subgroup）. The experimental group were suffered chronic unpredictable stress stimulations everyday. The control group drank and ate normally everyday. Rat was weighed every week, and the ethology performance scores of rats which evaluated by open-field box were recorded at the time of before experiment, after 3 weeks and 6 weeks respectively. Results The weight of rats in experimental group decreased more rapidly than those of rats in the control group. There were significant difference between control group and experimental group in the scores of ethology performance of rats（P〈0.05）.Conclusions Long-term chronic unpredictable stress stimulation could affect the general body state of rats, such as changing the behaviors, inhibiting the increase of weight,
出处
《中国医药指南》
2012年第21期20-22,共3页
Guide of China Medicine
关键词
应激
行为
心理学
Stress
Behavior
Psychology
