2006-2010年耐甲氧西林金黄色葡萄球菌的测定与耐药性变迁

Detection of methicillin-resistant Staphylococcus aureus and drug resistance during 2006-2010

目的了解2006-2010年医院耐甲氧西林金黄色葡萄球菌(MRSA)的检出率与耐药性变迁。方法采用VITEK-60全自动微生物分析仪对菌株进行鉴定及药敏检测,药敏结果使用WHONET软件进行分析。结果共检出金黄色葡萄球菌867株,45.4%来自于脓液/创面,其次是呼吸道标本占22.6%;2006-2010年MRSA检出率分别为28.7%、31.5%、39.2%、53.6%、44.6%;MRSA对利奈唑胺、万古霉素的敏感率为100.0%。结论临床医师在治疗由MRSA引起的感染时首选利奈唑胺、万古霉素,严格要求医护人员在为患者治疗前后手卫生,一旦检测到MRSA应加强隔离预防,控制在医院内的扩散。

OBJECTIVE To explore the detection rate and the change in drug resistance of methicillin-resistant Staphylococcus aureus during 2006--2010. METHODS VITEK 60 automated microbial analyzer, the identification and drug susceptibility testing of the isolates were performed, and the results of the drug susceptibility testing were analyzed using WHONET software. RESULTS A total of 867 strains of S. aureus were detected, 45.4%of the isolates were isolated from pus or wound, 22. 6% from respiratory tract specimens; the detection rates of methicillin-resistant S. aureus during 2006- 2010 were 28. 7%, 31. 5%, 39. 2%, 53. 6%, and 44. 6%, respectively; the susceptibility rates of MRSA to linezolid and vancomycin were 100.0%. CONCLUSION Linezolid and vancomycin should be taken as the first consideration for the treatment of MRSA infections, it is necessary to stress the importance of hand hygiene of the medical staff before and after treatment; once MRSA infection is detected, the isolation and prevention should be intensified to control the prevalence in hospital.