化学发光免疫分析法在检测儿童EB病毒感染中的应用

Detection of EB virus infections in children through chemiluminescent immunoassay

目的探讨化学发光免疫分析法检测EB病毒抗体在儿童临床EB病毒(EBV)感染诊断中的价值。方法对228例符合EB病毒感染诊断标准的患儿采用化学发光免疫分析法检测血浆样本中VCA-IgM、EA-IgG、EB-NA-IgG、VCA-IgG含量。结果血浆VCA-IgM、EA-IgG、EBNA-IgG、VCA-IgG的检出率分别为84.65%、20.61%、25.88%、76.75%,差异具有统计学意义(P<0.05);各种抗体以不同组合方式出现VCA-IgM阳性分类中,以VCA-IgM+VCA-IgG组合模式最为多见,VCA-IgM阴性分类中,则以VCA-IgG+EBNA-IgG组合模式最为多见;不同年龄段儿童VCA-IgM的检出率差异有统计学意义(P<0.05),初次感染的年龄段主要集中在0～3岁。结论化学发光免疫分析法检测EB病毒抗体对儿童EB病毒感染的明确具有早期、全面、快速等优点,适合临床检测。

OBJECTIVE To explore the clinical significance of chemiluminescent immunoassay in detection of Epstein-Barr virus infections. METHODS Plasma of 228 children with EB infections suited for diagnostics criteria was detected for VCA-IgM, EA-IgG, EBNA-IgG, and VCA-IgG by chemiluminescent immunoassay. RESULTS The positive rates of VCA-IgM, EA-IgG, EBNA-IgG, and VCA-IgG were 84. 65%, 20. 61%, 25. 88%, and 76.75 %, respectively, the difference was statistically significant （P〈0.05） ; among antibodies appeared as VCA- IgM positive classifications in a various combination way, VCA-IgM plus VCA-IgG positive was the most common; while among VCA-IgM negative, the VCA-IgG plus EBNA-IgG positive was the most common; the difference in the positive rate of VCA-IgM in the children of various age groups was statistically significant （P〈0.05） ; the primary infections mainly concentrated in the age group of 0-3 years of age. CONCLUSION EBV infections can be diagnosed in an early, comprehensive, and rapid manner by using chemiluminescent immunoassay which thus is suitable for clinical detection.