中药复方补剂和清剂对运动大鼠肾上腺c-fos蛋白表达的影响

The Effect of Chinese Herbal “Tonifying Prescription” and “Clearing Prescription” on c-fos in Adrenal Gland of Exercise Rats

目的 :了解补剂和清剂对运动大鼠肾上腺c-fos蛋白表达的影响。方法 :SD大鼠饲养、中药灌胃及运动训练 30天 ,第 31天进行规定时间游泳运动 ,取出半数大鼠予即刻处死、取出肾上腺及制片 (免疫组化法 ) ,剩余半数大鼠于休息 2 4小时后再予同法处死、取材及制片。结果 :游泳运动后即刻处死的三组大鼠间肾上腺c -fos蛋白表达量无明显差异 ,而游泳运动休息 2 4小时后清剂组较对照组的肾上腺c -fos蛋白的表达量明显降低。结论 :在对抗体力性疲劳的效应中 ,清剂有较好的对抗肾上腺伤害性刺激因素的作用。

Purpose To study the effect of Chinese herbal Clearing Prescription (CP) and Tonifying Prescription (TP) on c-fos expression in adrenal gland of exercise rats . Methods SD rats were fed and treated by herbs and exercised for 30 days. Half of them were killed immediately after swimming in a certain time at the 31st day. The remainings were killed 24 hours after exercise at the same day. Their adrenals were taken out and made in sections, then c-fos expression was analyzed with immunohistochemical method. Results There was not any difference of c-fos expression in adrenal among three group rats (herbal groups and control group) immediately after swimming. However, c-fos expression decreased significantly 24hrs after swimming in CP group as compared with control. Conclusion CP had a better affect of anti-physical fatigue on antagonizing the nocuous stimulation factors of adrenal.