骨肉瘤组织中p53蛋白表达与PCNA的相关性研究

INVESTIGATION ON THE EXPRESSION OF p53 PROTEIN AND PCNA IN OSTEOSARCOMA

目的 研究骨肉瘤组织中 p5 3蛋白表达与 PCNA的相关性。方法 应用免疫组化方法测定 33例骨肉瘤中p5 3基因、PCNA的表达 ,均行手术治疗 ,19例获得平均 1年7个月的随访 ,11例发生肺转移 (6例死亡 ) ,3例术后复发。结果 p5 3阳性表达率为 5 7.6 % ,预后良好组 p5 3阳性表达率明显低于预后不良组 ;PCNA阳性表达率为 6 6 .7%。结论 p5 3蛋白阳性表达的骨肉瘤有较强的增殖能力。

Objective To investigate the expression of p53 protein and PCNA and their relationship with the prognosis of patients with osteosarcoma.Methods In 33 cases of osteosarcoma, the immunohistochemical method was used to determine the expression of p53 protein and PCNA.All 33 cases were operated and 19 cases were followed up for a mean time of 1 year and 7 months postoperatively.Results Lung metastases occurred in 11 cases and in 3 cases the original sarcoma reccured.The positive expression rate of p53 protein was 57 6 % and this was apparently lower in the group of patients with better prognosis.The positive rate of expression of PCNA was 66 7 %.19 cases were followed up and 10 cases with positive PCNA expression showed bad prognosis.Conclusion Osteosarcoma patients with positive expression of p53 protein had stronger proliferation ability.$$$$