椰毒假单胞菌酵米面亚种鸟嘌呤和胞嘧啶含量的测定及2种测定方法的比较

Determination of guanine plus cytosine contentof Pseudomonascocovenenans subsp farinofermantans with two methods and the comparative study

利用复性速率法 (Tm值法 )和高效液相色谱法 (HPL C法 )对生化性状比较相近的椰毒假单胞菌酵米面亚种、唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌共 7个菌株的鸟嘌呤 (G)和胞嘧啶 (C)摩尔百分含量 (G+ C) %进行了测定 ,并对这 2种方法做了对比研究。结果发现 ,3个种的 7个菌株的 (G+ C) %比较接近 ,不能否定这 3个种的细菌属于同一个种。另外 ,HPL C法比 Tm值法的准确性好 。

The guanine plus cytosine content(G+ C) mol% of Pseudomonas cocovenenans subsp farinofermantans,Burkholderia gladioli and Burkholderia cocovenenans,which were similar in biochemical characteristics,were determined with Tm values m ethod and HPL C method,and the com parative study of these two methods was discussed. A negative conclusion could not be made from the results. The three bacteria were the same species.The HPL C method was more accurate than the Tm values method,but the repeatability of the latter was better.