167种食品中的李斯特菌检验

Identification of Listeria species in 167kinds of foods

通过对南京某地区 6类 16 7种食品李斯特菌的初步分离鉴定 ,发现 MMA分离平板上除 45°光线下为蓝色或灰蓝色的 S型小菌落外 ,呈现扁平、干燥、边缘不整齐的菌落 (R菌落 )也为李斯特菌的可疑菌落 ,同时发现触酶阴性、MR阴性、VP阴性及尿素酶阳性的菌株。结果表明 ,S型小菌落、尿素酶阴性、MR阳性。

Through preliminary identification of isolation of L isteria species in six types foods,which consisted of 16 7kinds of foods,we found both S- form and R- form colonies were suspected colonies of L isteria species,and som e of them were catalase- negative,urease- positive,methyl red(MR) negative and Voges- Proskauer(VP) negative.The results showed that S- form ,catalase- positive,urease- negative,MR- positive and VP- positive colony should not be used as the strict criteria for the identification of L isteria.