CD4、CCR5、CXCR4和DC-SIGN分子在HIV-1感染者及正常人晚孕胎盘及早孕绒毛的表达

Expression of CD4,CCR5,CXCR4 and DC-SIGN in chorionic villi and human placentae with or without HIV-1 infection

目的明确CD4、CCR5、CXCR4和DC-SIGN分子在HIV-1不同感染状态晚孕胎盘和早孕绒毛的存在及表达情况,为探索HIV-1宫内传播的分子机制提供理论依据。方法收集11例HIV-1感染孕妇胎盘、13例正常孕妇胎盘和10例早孕流产绒毛,免疫组化检测并比较3组孕妇胎盘或绒毛组织中HIV-1相关受体CD4、CCR5、CXCR4和DC-SIGN分子的存在情况及其表达强度。结果 CD4在早孕绒毛和晚孕胎盘中的表达存在个体差异,早孕组、对照组和病例组CD4阳性率分别为70.00%、61.54%、72.73%,3组阳性率差异无统计学意义(P=0.902)。CCR5、CXCR4和DC-SIGN分子在胎盘均有表达,均定位于滋养细胞和绒毛间质细胞和/或Hofbauer细胞,且在早孕绒毛中的表达强度均低于晚孕胎盘组,差异均有统计学意义(t1=-4.09,P1<0.001;t2=-4.80,P2<0.001;t3=-4.57,P3=0.001)。结论胎盘组织中存在多种HIV-1受体相关分子,具备感染的分子基础。胎盘滋养细胞上是否存在CD4分子具有个体差异;CCR5、CXCR4和DC-SIGN分子在晚孕胎盘中的表达高于早孕的绒毛,可能是HIV-1感染宫内传播多发生于孕晚期的原因之一。

Objective To investigate the expression of HIV-1 receptors CD4,co-receptors CCR5,CXCR4 and DC-SIGN in human placenta and chorionic villi and to explore the mechanism of in-utero transmission of human immunodeficiency virus type 1（HIV-1）.Methods 11 placentas from HIV-1 seropositive women,13 placentas from normal placentas and 10 cases of early pregnancy abortion villi were collected.Immunohistochemistry method was used to detect the expression of CD4,CCR5,CXCR4 and DC-SIGN.Results There were individual differences of CD4 expression in placenta,the positive rate of the three groups was 70.00%,61.54% and 72.73%,respectively.There was no statistical difference among three groups.All of CXCR4,CCR5,and DC-SIGN had positive expression in the placenta,they all located at the trophoblast cells and stromal of villi.The level of CXCR4,CCR5,and DC-SIGN expression in chorionic villi from first trimester were lower than those in placentas from the third trimester,the difference among the three groups was significant（t1=-4.09,P1〈0.001;t2=-4.80,P2〈0.001;t3=-4.57,P3=0.001）.Conclusions With the expression of CD4,CCR5,CXCR4 and DC-SIGN,placenta possessed the molecular basis of HIV-1 infection.There are individual differences in the expression of CD4 molecules in trophoblast cells.The expressions of CCR5,CXCR4 and DC-SIGN molecules in the placenta from the third trimester were higher than those in chorionic villi from first trimester,which might be related with the fact that most of MTCT occurred at the third trimester stage.