人脑胶质瘤尿激酶型纤溶酶原激活因子基因表达及意义

Expression and significance of urokinase-type plasminogen activator gene in human glioma

目的 探讨尿激酶型纤溶酶原激活因子 (uPA)基因在人脑胶质瘤中的表达及临床意义。方法 采用Northern印迹分子杂交和免疫组化方法检测 43例人脑胶质瘤和 5例正常脑组织uPAmRNA的蛋白表达 ,并与临床生物学参数进行综合分析。结果 上述组织或细胞均可表达 2 5Kb的uPAmRNA转录物 ,高级别胶质瘤较低级别和正常脑组织uPAmRNA表达水平明显增高 (P <0 0 1) ,低级别胶质瘤与正常脑组织的表达无显著差异 (P >0 0 5 ) ;术后 18个月内复发和生存期 <3年者uPAmRNA表达显著高于术后 18个月无复发及生存期 >3年者 (P <0 0 1) ,uPAmRNA表达水平与胶质瘤微血管数量呈显著正常相关 (r=0 5 6 ,P <0 0 1) ,与患者性别、年龄及肿瘤大小无显著相关 (P>0 0 5 )。uPA蛋白主要分布在胶质母细胞瘤、间变性星形细胞瘤的瘤细胞及内皮细胞 ,低级别胶质瘤较少。结论 uPA基因表达可能参与胶质瘤的侵袭、血管生成及恶性演变 。

Objective To investigate the expression and its clinical significance for urokinase type plasminogen activator(uPA) gene in human gliomas. Methods The mRNA level and protein expression of uPA were examined with Northern blot hybridization and immunohistochemical method in 43 cases of gliomas and 5 cases of normal brain tissues. Their relation to the clinical indexes was also comparetively analyzed. Results All tissues expressed 2 5kb transcripts of uPA mRNA. The uPA mRNA level in high grade glioma was considerably higher than those in low grade glioma and normal brains(P<0 01), but no significant difference was observed between low grade gliomas and normal brains tissues(P>0 05). Levels of uPA mRNA expression in tumor tissues of patients whose tumor recurred in 18 postoperative months and survival period were less than 3 years was significantly higher than counterparts(P<0 01). The uPA mRNA expression was considerably correlated with the microvessel quantity(MVQ) in gliomas(r=0 56,P<0 01) and had no significant correlation with sex or age of the patients and tumor size. The distribution of uPA protein was mainly in tumor cells and endothelial cells of glioblastomas and anaplastic astrocytomas, whereas it was almost unremarkable in low grade gliomas. Conclusions Expression of uPA gene may take part in glioma invasion, angiogenesis and progression of malignancy, and which may play a role in the recurrence and prognosis.