柱花草花粉活力的测定方法

Methods to Test Pollen Viability of Stylosanthes

利用6种常用花粉活力测定方法对柱花草花粉活力进行测定,并筛选出各方法的最佳配方。结果表明:以离体萌发法为最佳,最适培养基:10%蔗糖、10%PEG-6000(聚乙二醇)、0.01%H3BO3、0.03%CaCl2.2H2O、0.01%KNO3,pH 6.5,柱花草花粉在该培养基上30℃条件下培养10 min,萌发率可达84.03%。0.5%I2+1.5%KI的碘-碘化钾染色效果好,色差明显,较离体萌发法测定值偏高,但差异不显著,可选作简单快速测定法;FDA(荧光素二醋酸脂)染色法、醋酸洋红染色法、无机酸法、TTC(2,3,5-氯代三苯基四氮唑)法均不适合柱花草花粉活力的检测。

Used 6 commonly methods of pollen viability determination on Stylo pollen viability, and screened out their optimal formula. The results showed that pollen germination in vitro method was the best, and the best medium was 10% sucrose, 10% PEG-6000 （polyethylene glycol）, 0.01% HEBO3, 0.03% CaCl2, 2H2O, 0.01% KNO3 and pH 6.5, Stylo pollen germination in this medium at 30℃ for l0 min, and then pollen germination rate were 84.03%. It can be concluded that 0.5% I2 and 1.5% KI components of I-KI staining was the best staining method, because it got no significant difference viability rate to germination in vitro method and can dye fast and clearly. The FDA （fluorescein diacetate） staining, acetocarmine staining, inorganic acid method, TTC （2,3,5-Triphenyltetrazolium chloride ） staining were unsuitable to assay pollen vitality of Stylo.