Harpin类蛋白纳米粒的制备及促生作用

Preparation and Bioassay of Harpin Nanoparticles

HarpinXoo和HarpinXm分别来自水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)和棉花角斑病菌(Xanthomonas citri subsp.malvacearum,Xcm),都具有Harpin的特性。笔者将HarpinXoo和HarpinXm蛋白制成纳米粒剂型进行诱导烟草过敏性反应等研究,结果表明,这2种蛋白与其蛋白纳米粒都能在烟草叶片上激发过敏性反应和氧爆发现象,对烟草种子萌发具有促进作用,尤其HarpinXm蛋白纳米粒能更好地激发烟草产生氧爆发,促生效果达104.5%。HarpinXoo与HarpinXm蛋白纳米粒在释放的初始阶段即出现突释,至48h时分别达到83.9%和84.5%,起到持续性的促生效果。

Harpinx∞ and Harpinxm are derived from Xanthomonas oryzae pv. oryzae and Xanthomonas citri subsp. malvacearum, respectively, and have the characteristics of Harpin. Harpinx∞ and Harpinxm were prepared as nanoparticle agents. Both the two proteins and their protein nanoparticles induced hypersensitive response and oxidative burst in tobacco leaves, playing a beneficial role in the process of tobacco seed growing, especially Harpinxm nanoparticles which promoted oxidative burst of tobacco by 104.5%. Harpinx∞ and Harpinxm nanoparticles showed a burst release at the initial stage, and their release rates increased to 83.9% and 84.5%, respectively, within 48 h. These two nanoparticles played a role in promoting protein activity continuously.