艾灸对急性胃黏膜损伤大鼠HSP60、HSP70表达的影响

The effluence on impression of heat shock protein 60,heat shock protein 70 in acute gastric mucosal lesion after pro-moxibustion

目的观察艾灸中脘、足三里穴对急性胃黏膜损伤模型大鼠胃黏膜上皮HSP60、HSP70表达的影响。方法 32只SD大鼠完全随机分为空白组、模型组、艾灸穴位组和艾灸非穴位组。艾灸预处理大鼠8 d无水乙醇灌胃,造急性胃黏膜损伤模型。采用免疫组织化学法检测HSP60、HSP70的表达。结果与空白组比较,模型组中胃黏膜损伤指数、HSP60表达明显升高(P<0.01)。与模型组和艾灸非穴位组相比,艾灸穴位组胃黏膜损伤指数明显降低(P<0.01),HSP60、HSP70过度高表达(P<0.01)。结论艾灸中脘、足三里穴预处理能够降低无水乙醇对胃黏膜的损伤指数,诱导HSP60、HSP70的过度表达,减少伤害性刺激,从而起到保护胃黏膜的作用。艾灸穴位要比非穴位的作用更加明显,说明艾灸对胃黏膜保护作用具有一定的穴位特异性。

[Objective] To observe the effects of moxibustion at Zhongwan （RN12） - Zusanli （ST36） on apoptosis of gastric mucosal cells and the expressions of heat shock protein 60 （HSP60） and heat shock protein 70 （HSP70）. [Methods] 32 SD rats were randomly allocated to blank, model, moxibustion acupoint, moxibustion non-acupoint groups with 8 cases in each group. The rats were pre-treated with moxibustion for 8 days. Acute gastric mucosa injury model was established by anhydrous alcohol-induced method. Test apoptotic cells and expression of HSP60 and HSP70 by radio-immunity. [Results] Compared with blank group, the expression of gastric mueosal UI and HSP70 in model group increased significantly （P〈0.01）. While gastric mucosal UI decreased remarkably, HSP60 and HSPT0 were over expressed in moxibustion acupoint group compared with model and moxibustion non-acupoint group （P〈0.01, P〈0.01）. [Conclusion] Pretreatment of moxibustion at Zhongwan （RN12） - Zusanli （ST36） can reduce the injury of gastric mucosal caused by anhydrous alcohol, induce the over-expression of HSP 60 and HSP70, avoid noxious stimulus so as to protect the gastric mucosa which moxibustion acupoint was more potent compared with non-acupoint according to the result.