大鼠去大骨瓣减压术后脑组织S-100B蛋白表达变化的研究

Research on the changes of cerebral S-100B after decompressive craniotomy in rats

目的研究大鼠重型脑损伤后去大骨瓣减压组(DC组)和非去大骨瓣减压组(NDC组)S-100B的表达变化。方法运用免疫组织化学染色法检测假手术组(SH组)、NDC组和DC组在不同时间点S-100B蛋白的表达。结果 DC组与NDC组S-100B表达呈现双峰趋势,第1个峰值出现在伤后6 h,第2个峰值出现在伤后72 h,NDC组第2峰较高,DC组第2峰较低;NDC组在120 h及168 h S100B表达水平仍较高;NDC组在每个时间点的表达量均高于DC组,48、72、120和168 h 4个时间点比较差异有统计学意义(P<0.05)。结论去大骨瓣减压能够减轻继发性脑损伤。

[Objective] To research the expressions of S-100B between decompressive craniotomy group （group DC） and non decompressive eraniotomy group （group NDC） after severe brain injury in rats. [Methods] The expressions of S-100B protein were measured by immunohistoehemical staining in sham-operated group （group SH）, group NDC and group DC at different time points. [Results] There were two peaks for S- 100B expression in group NDC and DC. One peak appeared at 6 h after brain injury, the other appeared at 72 h. The second peak was higher than the first one in group NDC and contrary in group DC. Expression of S-IOOB was still high in group NDC at 120 h and 168 h. At any time point there were more positive expression in group NDC than DC and the differences were significant at 48h, 72h, 120h and 168h （P〈0.05）. [Conclusions] Decompressive eraniotomy could relieve secondary brain injury.