蒲黄黄酮对成年雌性大鼠雌孕激素及其受体的影响

Influence of Puhuang flavonoid on adult female rats' estrogen-progestin and its receptor

目的研究蒲黄黄酮雌激素样效应对成年雌性大鼠血清雌激素、孕激素含量及子宫组织中雌激素受体-α(ER-α)、雌激素受体-β(ER-β)和孕激素受体(PR)表达的影响。方法将SD大鼠60只随机平均分为五组:正常组、三苯氧胺+蒲黄黄酮组、蒲黄黄酮组、己烯雌酚组和三苯氧胺组。采用放射免疫法检测大鼠血清中雌二醇(E2)和孕酮(P)含量,免疫组化法检测各组大鼠子宫组织中雌激素受体-α(ER-α)、雌激素受体-β(ER-β)以及孕激素受体(PR)的表达水平。结果与正常组和三苯氧胺+蒲黄黄酮组相比,蒲黄黄酮组大鼠子宫内膜中ER-α、ER-β和P的阳性细胞表达及血液中E2和P的含量均显著增加(P<0.01);与己烯雌酚组相比,蒲黄黄酮组大鼠子宫内膜中ER-α、ER-β和P的阳性细胞表达及血液中E2和P的含量无明显变化,无统计学意义(P>0.05)。结论蒲黄黄酮能显著提高大鼠子宫内膜ER-α、ER-β和P的阳性细胞表达及血液中E2和P的含量,具有雌激素样效应。

【Objective】 Study on the influence of Puhuang flavonoid estrogen-like effects on the uterus of adult female rats,like this,E2,P in serum and the expression of ER-α,ER-β,PR in uterine tissues.【Methods】 SD rats（n=60） were randomly divided into 5 groups： Normal group（I）,Tamoxifen and Puhuang flavonoid group（II）,Puhuang flavonoid group（III）,Tamoxifen group（IV）,Tamoxifen group（V）.The content of E2,P in serum was detected by the method of immunoradiometric assay（IRMA）,while the expressions of ER,PR in uterine tissues were detected by the method of immunohistochemical staining（FIHC）.【Results】 Compared with normal group,Tamoxifen and Puhuang flavonoid group,the positive reaction cells of endometrial ER-α,ER-β and P,and blood content of E2 and P were increased significantly in Puhuang flavonoid group（P 〈0.01）;while by comparison with IV group,there was no significant change in the expression of endometrial ER-α,ER-β and P positive cells,and the blood content of E2 and P in flavonoid Puhuang rats（P 〉0.05）.【Conclusion】 Puhuang flavonoid with estrogen-like effects in rats can significantly improve the expression of endometrial ER-α,ER-β and P positive cells,as well as the blood content of E2 and P.