子宫肌瘤中IGF-Ⅱ、PCNA的表达及意义

The expression and clinical significance of IGF-Ⅱ and PCNA in Leiomyoma of Uterus

目的研究胰岛素样生长因子Ⅱ(IGF-Ⅱ)、增殖细胞核抗原(PCNA)在子宫肌瘤中的表达,探讨IGF-Ⅱ、PCNA在子宫肌瘤发生中的作用。方法用免疫组织化学方法,检测IGF-Ⅱ在子宫肌瘤组织、肌瘤旁平滑肌组织及正常子宫平滑肌组织中的表达,并用同法检测组织中PCNA的表达。结果①IGF-Ⅱ在子宫肌瘤组织、子宫肌瘤旁平滑肌组织及正常平滑肌组织中表达的阳性率分别为60%、16.67%、10%,三组表达率差异有显著性(P=0.000)。②PCNA在子宫肌瘤组织、子宫肌瘤旁平滑肌组织及正常平滑肌组织中表达的阳性率分别为83.3%、26.7%、20%,三组表达率差异有显著性(P=0.000)。③子宫肌瘤组IGF-Ⅱ的表达与PCNA的表达相关性研究rs=0.55,呈正相关,P<0.05,有统计学意义。结论①IGF-Ⅱ及PCNA在子宫肌瘤细胞中高表达,提示IGF-Ⅱ及PCNA可能参与子宫肌瘤的发生。②IGF-Ⅱ与PCNA在子宫肌瘤中的表达呈正相关,提示IGF-Ⅱ通过其促进细胞增殖的作用参与了子宫肌瘤的发病。

【Objective】 To determine the expression of insulin-like growth factor-Ⅱ（IGF-Ⅱ） and proliferating cell nuclear antigen（PCNA） in the uterine leiomyoma,and to investigate the role of IGF-Ⅱand PCNA in the pathogenesis of uterine leiomyoma.【Methods】 Immunohistoehemical method was employed to detect the expression of IGF-Ⅱin the uterine leiomyoma,the tissues beside hysteromyoma and the normal myometrium,the same methods to detect the expression of PCNA.【Results】 ①The expression of IGF-Ⅱ： The positive expression rates in the uterine leiomyoma tissues beside hysteromyoma and the normal myometrium were 60%,16.67%,10%.There were significant differences between the uterine leiomyoma,the tissues beside hysteromyoma and the normal myometrium（P =0.000）.② The expression of PCNA： The positive expression rates in the uterine leiomyoma,the tissues beside hysteromyoma and the normal myometrium were 83.3%,26.7%,20%.There were significant differences between the uterine leiomyoma,the tissues beside hysteromyoma and the normal myometrium（P =0.000）.③The expression of IGF-Ⅱ and PCNA was positive correlation（rs =0.55,P 〈0.05）.【Conclusion】 ①The high expression of IGF-Ⅱand PCNA in uterine leiomyoma indicates IGF-Ⅱand PCNA are related with the development of the uterine leiomyoma.②The positive correlation between IGF-Ⅱand PCNA suggest that IGF-Ⅱrelated with cell proliferation and this relationship maybe one of the etiology of the Uterine leiomyoma.