毛冬青及其同属药用植物高效薄层色谱指纹图谱研究

Study on HPTLC Chromatographic Fingerprint Analysis of Ilex pubescens and Its Related Species

目的以毛冬青为基础建立毛冬青三萜类化合物高效荧光薄层色谱指纹图谱,并与冬青属岗梅,救必应等常用药用植物指纹图谱进行比较分析。方法硅胶GF254高效预制薄层板,以氯仿-乙酸乙酯-甲醇-水-甲酸(3∶22∶5∶2∶0.2)为展开剂展开,10%硫酸乙醇溶液显色后,获得薄层荧光色谱指纹图谱,经指纹图谱系统解决方案软件(Chromafinger2005)生成共有模式,进行测试与分析。结果毛冬青薄层荧光色谱指纹图谱由13个特征荧光条斑组成。荧光色谱图像与轮廓扫描图谱结合分析比较,建立毛冬青指纹图谱模式。毛冬青不同部位,以及与同属常用药用植物之间在三萜类化合物方面区别较大。结论通过薄层荧光指纹图谱的建立,能快速有效区分毛冬青不同部位,及同属混伪品。

OBJECTIVE To develop the high-performance thin-layer chromatographic （ HPTLC ） fingerprints of triterpenoid from flex pubescens and compare the fingerprints similarity with its related species, such as flex rotunda and llex asprella. METHODS The separation was performed on pre-coated HPTLC GF254 silica-gel plate stationary phase with trichloromethane-ethyl acetate-methanol- water-formic acid （3： 22： 5： 2：0. 2） as solvent system and 10% sulfuric acid alcoholic solution as color development reagent. The com- mon pattern of HPTLC fingerprints were obtained through Chromafinger2005 solution software, and authentication and quality assess- ment were analyzed by similarity and Principle Commonent Analysis. RESULTS The common pattern of the pileus of flex pubescens consists of 13 characteristic peaks. There are notable differences among the fingerprints of different parts of flex pubescen, and those be- tween flex pubescens and its related species. CONCLUSION The different parts of Ilex pubescens can be distinguished by compari- son of the HFFLC chromatographs. The other relatives can be easily differentiated from flex pubescens too.