长梗喉毛花正丁醇部位化学成分研究

Chemical constituents of n-BuOH extract of Comastoma pedunculatum

通过硅胶、大孔树脂、葡聚糖凝胶Sephadex LH-20和HPLC等多种色谱分离方法相结合,从长梗喉毛花Comas-toma pedunculatum(Royle ex D.Dou)Holub全草乙醇提取物的正丁醇萃取部位中分离得到13个化合物,其中9个皂苷类,4个黄酮碳苷类;根据化合物的理化性质和波谱数据鉴定化合物的结构为柴胡皂苷元F(1),3-O-β-D-呋糖基柴胡皂苷元F(2),风轮菜苷ⅩⅤ(3),柴胡皂苷A(4),6″-乙酰基柴胡皂苷A(5),风轮菜苷Ⅰ(6),柴胡苷Ⅰ(7),风轮菜苷Ⅻ(8),柴胡皂苷b3(9),异牡荆苷(10),当药黄素(11),异荭草素(12),3',4',5-三羟基-7-甲氧基-6-C-β-D-吡喃葡萄糖黄酮苷(13)。化合物1～10,12,13均为首次从该属植物中分离得到。

Thirteen compoumds were isolated from the n-BuOH portion of the 70% ethanolic extract of Comastoma pedunculatum by a combination of various chromatographic techniques including silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC, of which nine were triterpenoid saponins and four were flavone C-glycosides. Their structures were elucidated by spectroscopic data as saikogenin F （ 1 ）, 3-O-β-D-fucopyranosylsaikogenin F （2）, clinoposaponin X V （3）, saikosaponin A （4）, 6＂-acetylsaikosaponin A （5）, clinoposaponin I （6）, bupleuroside I （7）, clinoposaponin Ⅻ（8）, saikoponin b3 （9）, isovitexin （10）, swertisin （ U ）, isoorientin （ 12）, 3＇ ,4＇,5-trihydroxy-7-methoxy-6-C-β-D-glucopyranosyl-flavone （ 13 ）. Compounds 1-10, 12-13 were all isolated from Comastoma genus for the first time.