摘要
目的:研究芍药苷对Aβ25-35诱导的PC12细胞损伤的保护作用。方法:用Aβ25-35造成PC12细胞损伤模型,采用MTT比色法检测细胞存活率,Annexin-V/PI双染流式细胞计量法检测细胞凋亡,JC-1和Fluo-3荧光探针法分别观察线粒体膜电位和细胞内钙离子变化,Western印迹法检测Bcl-2,Bax和Caspase-3蛋白的表达水平,观察芍药苷对PC12细胞损伤的保护作用。结果:芍药苷可抑制Aβ25-35所诱导的PC12细胞毒性和凋亡,并呈剂量相关性,其保护作用可能是通过提高线粒体膜电位、降低Ca2+浓度;增加Bcl-2蛋白表达、降低Bax蛋白表达、抑制凋亡相关蛋白Caspase-3的激活而实现的。结论:芍药苷对Aβ25-35诱导PC12细胞损伤具有保护作用。
Objective： To investigate the protective effect of paeoniflorin on PC12 cell injury induced by Afl25-35. Method： Aβ25-35-induced PC12 cell injury model was established. The effect of cell proliferation was detected by the MTT method. We also measured the apoptosis with the FITC-Annexin V/PI staining, observed the mitochondrial membrane potential and changes in intracel- lular calcium using JC-1 and Fluo-3/AM staining respectively, and studied the protein expression of Bax, Bcl-2, cleaved Caspase-3 by Western blotting analysis. Result： Paeoniflorin could inhibit Aβ25-35-induced PC12 cytotoxicity and apoptosis and show dose dependence. Its protective effect may be realized by increasing mitochondrial membrane potential Bcl-2 protein expression, decreasing Ca2＋ concentration and Bax protein expression and inhibiting activation of apoptosis-related protein Caspase-3. Conclusion： Paeoniflorin shows a protective effect on Aβ25-35-induced PC12 cell injury.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第16期2448-2451,共4页
China Journal of Chinese Materia Medica
基金
河南省医学科技攻关计划项目(201003079)
