金丝桃苷的大鼠胚胎-胎仔发育毒性研究

Study on toxicity of hyperoside in rat embryo-fetal development

目的:观察金丝桃苷对Wistar大鼠的胚胎-胎仔发育毒性,为妊娠期安全用药提供参考。方法:取健康受孕大鼠,随机分为金丝桃苷低、中、高(30,175,1 000 mg.kg-1)剂量组、阳性对照组(环磷酰胺,7 mg.kg-1)和溶媒对照组(等体积1%羧甲基纤维素钠水溶液)。分别于母鼠受孕后第6～15天灌胃给予1%羧甲基纤维素钠水溶液和金丝桃苷,环磷酰胺组于母鼠受孕后第11～13天皮下注射给药。给药后观察孕鼠饮水、摄食、生长等一般状况,妊娠第20天处死孕鼠。观察母体动物的临床症状、体重变化、黄体数、着床数、胚胎生存死亡情况、生存胎仔的体重、性别、外观、内脏及骨骼检查等指标。结果:金丝桃苷对各给药组的母鼠和胎鼠的外观、内脏及骨骼发育均未见明显异常。但是金丝桃苷高剂量组可致胎鼠体重增长减缓,身长和尾长缩短(P<0.05)。结论:在本实验条件下,金丝桃苷对胎鼠生长发育有一定影响,建议临床用药时怀孕妇女应慎用。

Objective ： To observe the toxicity of hyperoside in rat embryo-fetal development, in order to provide preference for safe use of drugs during gestation period. Method： Healthy pregnant rats were randomly divided into hyperosid groups （30, 175,1 000 mg . kg- 1 d - 1 ）, the positive control group （ cyclophosphamide, 7 mg . kg-1 d- 1 ） and the solvent control group （ 1% aqueous carboxymethylcellulose）. These rats were orally administered with hyperosid or vehicle during 6-15 d after gestation and subcutaneously injected with cyclophosphamide during 11-13 d. Maternal clinical sign, abortions, premature deliveries and body weight were monitored throughout gestation. At termination （gestation days 20）, pregnant females were evaluated for clinical symposiums, weight change, corpora lutea count, existence and death of embryos; live fetuses were examined for gender, external, visceral and skeletal malformation and variations. Result： All pregnant rats showed no significant abnormality in appearance, viscera and skeletal development. However, there was a difference between the high-dose group of hyperoside and negative control group in the fetus body weight, the length of the embryos and the length of tail （P 〈 0. 05 ）. Conclusion ： Pregnant women are suggested to cautiously use hyperoside because it shows certain impact on development of fetal rats under the experimental conditions.