康艾注射液对大鼠肝纤维化的影响

Effect of Kang′ai injection on hepatic fibrosis in rats

目的:探讨康艾注射液抑制大鼠肝纤维化的作用。方法:选取雌性Wistar大鼠30只随机分成康艾注射液干预组、单纯造模组和空白对照组各10只,实验组以含0.01%二乙基亚硝胺(diethylnitrosamine,DEN)饮水为纤维化诱导剂,干预组在造模过程中应用康艾注射液(1 mL.kg-1,腹腔注射,每周1次)治疗,对照组腹腔注射等量生理盐水作对照,空白对照组给予正常食水作对照。大鼠在同样条件下饲养21周观察HE染色和VG特殊染色下肝脏组织的病理形态学变化。结果:HE染色后单纯对照组镜下见正常肝小叶结构破坏,被假小叶取代,可见较多硬化结节,而干预组的肝小叶结构破坏相对较轻,可见较少硬化结节;VG染色后,空白对照组的纤维化程度为(6.52±2.64)%,康艾注射液干预组的纤维化程度明显低于单纯造模组[(17.41±1.11)%vs(20.18±2.52)%],3组相比均有统计学意义(P<0.05)。结论:在DEN制备的大鼠肝纤维化模型中,康艾注射液在一定程度上可抑制肝纤维化的生长。

Objective： To investigate the inhibition role of Kang＇ai injection （KAI） in rats with hepatic fibrosis. Method： Animal model with liver fibrosis were induced by 0. 01% concentration of diethylnitrosamine （DEN）. 30 female Wistar rats （ 160-200 g） were randomly divided into 3 groups：the KAI-DEN group, the DEN group and the blank control group. The KAI-DEN group was administered Kang＇ai injection （ 1 mL . kg-1 , intraperitoneal injection, once a week） and the DEN group was administered normal saline intraperitoneal injection. HE staining and VG special staining of liver tissue were used to evaluate liver fibrosis. Result： Compared with the DEN group, relatively less structural damage and less pseudolobular formation in the KAI-DEN group. Collagen area of the blank control group, the KAI-DEN group and the DEN group were （6. 52 ± 2.64） %, （ 17.41 ± 1.112） % and （20. 180 ± 2.519） %, respectively. The difference was statistically significant, P 〈 0. 05. Conclusion： Kang＇ai injection could inhibit the formation of DENinduced liver fibrosis.