一种生物素产生菌的简便快速平板筛选方法

A Convenient and Quick Method for Screening Biotin-producing Bacteria

介绍一种简便快速筛选生物素产生菌的方法,该方法运用微生物生长圈法原理,以生物素营养缺陷型菌株为指示菌。制作混有该指示菌的缺乏生物素的培养基单层平板,将土壤样品稀释涂布在此平板上培养,所形成的菌落如果分泌生物素到培养基中,菌落周围就会产生指示菌的生长圈,由此可初步获得生物素产生菌。实验利用初筛和复筛并结合管碟法检测,从大量土壤样品中筛选产生物素能力较强的菌株,运用高效液相色谱法验证,确定该筛选方法有效可靠。

This paper introduced a convenient and quick method to screen biotin-producing bacterium according to the bacterial growth circle method. The biotin auxotrophie strain was used as indicator bacteria and soil sample was spread onto biotin-deficient single-level plate which contained instructor bacteria. The indicator bacteria would grow a- round the biotin-producing bacterium. According to the pre-sereening method and re-screening method along with cyl- inder-plate method, we could obtain several strains which have relatively higher ability to produce biotin. The HPLC was further applied to confirm the production of biotin, meanwhile to confirm the validity of the screening method.