生物信息学预测hsa-miR-26b的作用靶标及功能

Targets and function of hsa-miR-26b predicted by bioinformatics

目的通过生物信息学预测hsa-miR-26b的靶基因,进一步分析其潜在功能,为其后续功能研究提供理论依据。方法用pubmed、谷歌(google)等工具检索hsa-miR-26b相关文献;用miRbase、NCBI、UCSC Browser和Promoter scan等在线工具分析hsa-miR-26b序列及所在基因组特征;用TargetScan、PicTar及miRanda预测hsa-miR-26b的靶基因;通过功能富集分析(GOanalysis)和信号转导通路富集分析(Pathway analysis),预测所得靶基因和已证实的靶标基因。结果研究证实hsa-miR-26b与神经发育、心肌肥大及各种肿瘤发生、发展有关;hsa-miR-26b位于CTDSP1基因内含子内,但可能具有与该基因不同的启动子;3种方法预测结果取交集获得可能靶标基因33个,合并已知靶标基因14个;hsa-miR-26b靶基因主要参与钠离子转运、溶酶体组织和生物形成、抑制细胞增殖、转运等生物学过程,涉及Notch、氨基糖类代谢、ABC转运子、VEGF及TGF-β等信号转导通路。结论 hsa-miR-26b的功能较为广泛,与发育、代谢等密切相关。

Objective To predict the target genes and function of hsa-miR-26b by bioinformatics analysis, and provide the theoretical basis for the further research. Methods The literatures related to hsa-miR-26b were searched by PubMed and Google tools. The sequence and genome characteristics of hsa-miR-26b were analyzed on line by miRbase database, NCBI, UCSC Browser and Promoter scan. The target genes for hsa-miR-26b were predicted by TargetScan, PieTar and miranda, and the function of the target genes were demonstrated by Gene Ontology Enrichment（GO） and signal transduction pathway analysis. Results Hsa-miR-26b related to cardiac hypertrophy, neurogenesis and tumorigenesis. Hsa-miR-26b was located within the intron of CTDSP1 gene, but might have a promoter independent of CTDSP1. By searching the literatures and finding the common target genes from TargetScan, PicTar and miranda, 33 predicted targets and 14 known targets were obtained. GO analysis showed that the target genes of hsa-miR-26b might participate in the biological processes of sodium ion transport, lysosome organization and biogenesis, negative regulation of cell proliferation and trans- port. The pathway analysis showed that these target genes were mainly involved in Notch, aminosugars metabolism, ABC transporters, VEGF and TGF-beta signaling pathways. Conclusion Hsa-miR-26b may have extensive functions and closely relate to development, metabolism and etc.