N-乙酰半胱氨酸对辐照引起的大鼠肠隐窝上皮细胞损伤的减轻作用

The therapeutic efficacy of N-acetylcysteine on γ-radiation-induced intestinal epithelial cell damage in rats

目的研究N-乙酰半胱氨酸(NAC)对辐照引起的肠隐窝上皮细胞IEC-6的损伤的防护作用及可能机制。方法将IEC-6细胞分为3组:正常组(无辐照),辐照组(6 Gy辐照)和NAC治疗组(6 Gy辐照+NAC 10 mg/mL)。IEC-6细胞辐射损伤模型采用总剂量为6 Gy的钴-60照射,用DCFH-DA测定细胞内的活性氧(ROS)和丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)的活力,用Annexin V-FITC和碘化丙啶进行细胞凋亡的检测,同时用Hoechst 33342进行细胞凋亡的检测,同时进一步检测IEC-6细胞的增殖能力。结果 NAC减少了辐照引起的ROS和MDA的过量产生以及SOD的过度消耗,同时流式细胞仪和核染色的结果显示,NAC抑制了辐照引起的细胞凋亡和增殖活力的下降。结论 NAC可以减轻辐照引起的IEC-6细胞的损伤。

Objective To investigate the protective effect of N - acetylcysteine on γ- radiation - induced intestinal epithelial cell （IEC - 6） damage in rats. Methods IEC - 6 ceils were divided into three groups ： normal group （Group N）, radiation group （6 Gy radiation, Group R） and NAC treatment group （6 Gy radiation ＋ NAC （10 mg/mL）, Group E）. Reactive oxygen species （ROS） in cultured IEC -6 cells was measured using Fluorescent Probe 2＇, γ -dichlorofluorescin diacetate （ DCFH - DA）. Malonaldehyde （MDA） and superoxide dismutase （SOD） assays were also performed at 24hrs post- radiation. Cell apoptosis was determined by annexin V- APC and propidium iodide （PI） stain u- sing the Apoptosis Detection Kit. Furthermore, the cells were stained with Hoechst 33342 （apoptotic cell） to visualize the nuclei. Alamarblue cell viability reagent was used to determine the cell viability exposure to T -radiation. Results NAC reduced the γ- radiation - induced over - production of ROS and MDA, and maintained the SOD activity in IEC - 6 cells. In addition, the radiation - induced apoptosis and cell viability reduction was alleviated by NAC treatment. Conclusion NAC could significantly reduce γ -radiation- induced IEC -6 damage.