超声弹性成像监测下大鼠肝纤维化模型的制备

The construction of liver fibrosis rat model under ultrasound elastography monitoring

目的超声弹性成像监测下使用硫代乙酰胺诱导大鼠肝纤维化模型,以提高成模率和成模一致性。方法 30只雄性SD大鼠随机分为3组,每组10只。第1组正常喂养作为正常对照组;第2组为传统建模组,即硫代乙酰胺(TAA)皮下注射200 mg/kg,每周2次,连续注射6周,第3组为监测建模组,即从第2次注射开始,每次注射前使用超声诊断仪进行检查,并根据检查结果决定该次是否进行注射。结果监测建模组大鼠死亡率为10%,肝纤维化形成率为90%;传统建模组大鼠的死亡率为30%,肝纤维化形成率为60%。两组差异有统计学意义(P<0.05)。结论肝纤维化诱导过程中根据超声弹性成像结果调整TAA的诱导剂量,可成功诱导大鼠肝纤维化,降低大鼠死亡率。

Objective To evaluate the ultrasound elasticity imaging monitoring in construction of thioacetamide - induced hepatic fibrosis rat model Methods Thirty male SD rats were randomly divided into three groups （ n = 10 ） ： control group （ Group 1 ）, traditional group （ Group 2, tradition TAA was given） and ultrasound elastography （UEG） monitoring group （ Group 3, TAA was given according to the UEG results）. Results There was significant reduction of mortality and improvement of fibrosis rate in Group 3 when comparing with those in Group 2 （ 10% vs 30% and 90% vs 60%, respectively, P 〈 0. 05 ）. Conclusion UEG guidance construction of hepatic fibrosis rat model is feasible with high successful rate and low mortality.