摘要
目的超声弹性成像监测下使用硫代乙酰胺诱导大鼠肝纤维化模型,以提高成模率和成模一致性。方法 30只雄性SD大鼠随机分为3组,每组10只。第1组正常喂养作为正常对照组;第2组为传统建模组,即硫代乙酰胺(TAA)皮下注射200 mg/kg,每周2次,连续注射6周,第3组为监测建模组,即从第2次注射开始,每次注射前使用超声诊断仪进行检查,并根据检查结果决定该次是否进行注射。结果监测建模组大鼠死亡率为10%,肝纤维化形成率为90%;传统建模组大鼠的死亡率为30%,肝纤维化形成率为60%。两组差异有统计学意义(P<0.05)。结论肝纤维化诱导过程中根据超声弹性成像结果调整TAA的诱导剂量,可成功诱导大鼠肝纤维化,降低大鼠死亡率。
Objective To evaluate the ultrasound elasticity imaging monitoring in construction of thioacetamide - induced hepatic fibrosis rat model Methods Thirty male SD rats were randomly divided into three groups ( n = 10 ) : control group ( Group 1 ), traditional group ( Group 2, tradition TAA was given) and ultrasound elastography (UEG) monitoring group ( Group 3, TAA was given according to the UEG results). Results There was significant reduction of mortality and improvement of fibrosis rate in Group 3 when comparing with those in Group 2 ( 10% vs 30% and 90% vs 60%, respectively, P 〈 0. 05 ). Conclusion UEG guidance construction of hepatic fibrosis rat model is feasible with high successful rate and low mortality.
出处
《广东医学》
CAS
CSCD
北大核心
2012年第16期2376-2378,共3页
Guangdong Medical Journal
基金
广东省自然科学基金资助项目(编号:10151006001000013)
广州市医药卫生科技项目(编号:20121A011027)
关键词
肝纤维化模型
超声弹性成像
硫代乙酰胺
hepatic fibrosis model
ultrasound elasticity imaging
thioacetamide