摘要
本研究从体内和体外两方面探讨了川芎嗪对肺气肿的防治作用。体外试验发现川芎嗪对弹性蛋白酶(简称:弹性酶)有明显的抑制作用,治疗剂量可取代血清防治肺气肿。体内实验是以弹性酶肺气肿动物模型观察血清弹性酶抑制力(SEIC)正常的血清和川芎嗪对弹性酶在肺内的积聚及其活性的影响;在光镜和电镜下对肺组织形态加以测量,用体视学方法分析。结果表明实验防治组未出现模型组的病理变化,与生理盐水对照组相近。用气溶胶法制成豚鼠弹性酶肺气肿模型并用川芎嗪防治有效,为探讨人类肺气肿发生机制和防治提供了实验依据。
This study demonstrated that ligustrazine possesses inhibitory effect obviously on elastic enzyme in vitro,and it can be used to prevent and treat emphysema instead of serum.Using light microscope and electron microscope,the authors observed morphological indexes and analyzed the indexes with stereology and statistics in lung tissues of guinea pigs.The results showed that there was no obvious difference between the ligustrazine administration group and the saline control group as pathological changes were not found in the Iigustrazine administrasion group under microscope. The shapes of elastic proteins were the same under electron microscope observation.Aerosol inhalational method induced emphysema model of elastic enzyme in guinea pig could be improved with ligustrazine treatment.
