小白菊内酯对细菌脂多糖致大鼠学习记忆功能减退的保护作用

The effect of parthenolide on cognitive dysfunction induced by intra-peritoneal LPS injection in rats

目的探讨小白菊内酯对腹腔注射细菌脂多糖(LPS)致大鼠学习记忆功能减退的改善作用。方法健康成年雄性Wistar大鼠36只,随机分为3组,每组12只。对照组腹腔注射生理盐水,致炎组腹腔注射LPS(10mg/kg),治疗组腹腔注射LPS(10mg/kg)和小白菊内酯(5mg/kg)。依据测试时间不同,每组再分为两个亚组,近期组在LPS注射后第3天开始实验;远期组在LPS注射后第12天开始实验。应用Morris水迷宫实验测试系统,采用定位航行实验和空间探索实验测试大鼠认知功能改变。结果炎症近期,与对照组和治疗组相比,致炎组寻台持续时间显著延长(P<0.05),第二象限停留时间和路程显著缩短,第一次穿越时间显著延长,穿越次数显著减少(P<0.05);炎症远期,3组动物学习记忆能力差异无统计学意义,各组游泳速度差异无统计学意义。结论小白菊内酯可改善腹腔注射LPS近期大鼠学习记忆功能减退。

Objective To observe the effect of parthenolide on cognitive dysfunction induced by intra-peri- toneal LPS injection in rats. Methods An animal model was established by intra-peritoneal LPS （10 mg/kg） injection. Thirty six rats were randomized into three Groups： Control Group（A）, LPS injection（10 mg/kg） Group（B）, LPS （10 mg/kg）and Parthenolide（5 mg/kg） injection Group（C）. The behaviors of animals were tested with Morris water maze respectively at the third day and the twelfth day after LPS injection. Results In short-term experiment, parthenolide greatly improved im- pairement of learning and memory dysabilities induced by LPS injection, while there was no significant difference between groups in long-term experiment. Conclusion Parthenolide may be useful to improve the short-term cognitive dysfunction induced by intra-peritoneal LPS injection.