血管内皮生长因子在火针治疗褥疮小鼠创面中的表达及作用

Expression and Effect of Vascular Endothelial Growth Factor in the Wound Surface in Fire Acupuncture Treatment of Bedsore Mice

目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)在火针治疗褥疮小鼠创面中的表达与作用。方法清洁级健康雌性SD小鼠72只随机分为对照组、红外线组、普针组和火针组,建立小鼠褥疮模型,分别于第2 d及治疗后3、7、10、15 d取材病理检测,HE染色观察组织学变化。通过免疫组化结合图像分析等技术,观察各组小鼠创面肉芽组织中VEGF表达情况和微血管的数目(microvessel count,MVC)。结果经单因素方差分析及两两比较,可以认为火针组VEGF表达及微血管数目与对照组、普针组及红外线组有差别(P<0.05,P<0.01),普针组与红外组疗效无差别(P>0.05)。结论血管内皮生长因子在火针治疗褥疮小鼠创面中的表达明显高于普针组、红外线组及对照组。

Objective To investigate the expression and effect of vascular endothelial growth factor （VEGF） in fire acupuncture treatment for the wound surface in bedsore mice. Method Seventy-two clean-class healthy female SD mice were randomly assigned to control, far-infrared, conventional acupuncture and fire acupuncture groups. A mouse model of bedsore was made. The tissue was taken for a pathological examination at the second day, and 3, 7, 10 and 15 days after treatment. Histological changes were observed by HE staining. VEGF expression and microvessel count （MVC） in the granulation tissue of wound surface were observed by an immunohistochemical method and image analysis in various groups of mice. Result It showed by one-way ANOVA and pairwise comparison that, the expression of VEGF and MVC in fire acupuncture group were significantly different from those in conventional acupuncture group and far-infrared group （P〈0.05,P〈0.01）, and the difference in therapeutic efficacy between conventional acupuncture and far-infrared groups was insignificant （P〉0.05）. Conclusion The expression of vascular endothelial growth factor in the wound surface is significantly higher in the fire acupuncture group of bedsore mice than in the conventional acupuncture, far-infrared and control ~roups.