摘要
本文选用 ~aH-TdR 掺入法测定脾淋巴细胞的增殖,DPH 标记淋巴细胞膜,荧光偏振法测定脾淋巴细胞和骨髓细胞的荧光偏振度。结果:DBA 纯系健康对照小鼠脾淋巴细胞增殖高,萤光偏振度较大,脂质流动性小;L_(1210)腹水癌小鼠脾淋巴细胞增殖大大减少,荧光偏振度减小,膜脂质流动性增大。0.4g/kg 脾肾方分别给健康和 L_(1210)小鼠灌胃9天后,脾肾方可使脾淋巴细胞增殖升高,并能调节膜脂质流动性接近于健康小鼠。对骨髓细胞膜脂质流动性也有相同作用。初步揭示了脾肾方对免疫不平衡动物 T 细胞功能的调节作用和骨髓细胞脾淋巴细胞膜脂质流动性相关的机制。
Proliferation of Con A stimulated splenic lymphocyte was examed by incorporation of ~3H- thymidine.Fluorescence polarization of DPH labelled splenic lymphocyte and bone marrow cells was measured.Proliferation of Con A stimulated splenic 1 mphocyte in DBA inbred healthy mice was higher than that of L_(1210)mice but fluorescence polarization of DPH labelled splenic lymphocyte in same healthy mice was lower than that of L_(1210)mice.ie.membranous lipid lymphocyte fluidity of lymphocyte in the healthy mice was smaller than that of L_(1210)mice.9 days after administration of Pi-Shen recipe by tube stomach proliferation of Con A-lymphocyte in the healthy mice has been increased.The recipe-adjusted splenic lymphocyte membrane lipid fluidity of L_(1210)mice to level of those in healthy mice.Effects of pi-Shen recipe on lipid fluidity of bone marrow cell membrane of L_(1210)mice were almost similar to that in splenic lymphocytes.These studies suggest that the mechanism on adjustable role of Pi-Shen recipe on T-lymphocyte function related to lymphocyte membrane lipid fluidity.
