CS_2吸入染毒对雄性大鼠下丘脑-垂体-性腺轴超微结构的影响及NO干预作用

Ultrastructural Changes of Hypothalamus-Pituitary-Gonad Axis after Inhalation of Carbon Disulfide in Male Rats and The Intervention of Nitric Oxide

[目的]探讨二硫化碳(CS2)对雄性大鼠下丘脑-垂体-性腺轴超微结构的影响及一氧化氮(NO)的干预作用。[方法]将24只雄性SD大鼠按体重随机分为4组:对照组、CS2染毒组、硝普钠(sodium nitroprusside,SNP)干预组和N-甲基-L-精氨酸(L-NMMA)干预组。除对照组外,其余3组均以1250mg/m3CS2进行静式吸入染毒,2h/d,5d/周,共10周,SNP和L-NMMA干预组在动物染毒结束前10d开始分别腹腔注射SNP(5mg/kg)和L-NMMA(2mg/kg),1次/d。染毒结束后,取下丘脑、垂体和睾丸组织,利用透射电子显微镜观察组织超微结构的改变。[结果]CS2染毒可造成下丘脑神经元、垂体促性腺激素细胞、生长激素细胞和睾丸支持细胞线粒体肿胀,内质网扩张,SNP对CS2引起的下丘脑、垂体、睾丸组织的损伤具有拮抗作用,而L-NMMA则进一步导致病变的发生。[结论]CS2可造成下丘脑、垂体和睾丸组织超微结构的改变,NO在此过程中发挥重要作用。

[Objective] To study the ultrastructural changes of hypothalamic-pituitary-gonadal axis（HPGA） in carbon disulfide（CS2） treated rats and the interventional effect of nitric oxide（NO）.[Methods]Twenty-four Sprague-Dawley male rats were randomly divided into 4 groups as： control,CS2,sodium nitroprusside（SNP） intervention and N-methyl-L-arginine（L-NMMA） intervention groups.Three experimental groups were treated with 1250mg/m3 CS2 by inhalation for 10 weeks,2h/d,5d/w.Two intervention groups received intraperitoneal injection of SNP（5mg/kg）,a nitric oxide donor,and L-NMMA（2mg/kg）,a nitric oxide synthase inhibitor,once a day for 10 days before the end of CS2 exposure.Then ultramicropathology technique was used to observe the ultrastructure of hypothalami,pituitaries and testes isolated from rats.[Results]CS2 induced swelling of hypothalamic neurons,gonadotropin cells,growth hormone cells and sertoli cells in male rats as well as widened endoplasmic reticulum.SNP reduced the effects of CS2 on ultrastructural changes;however L-NMMA enhanced the effects.[Conclusion]CS2 could induce ultrastructural changes of hypothalamus,pituitary and testis in male rats,which is mediated through NO.