摘要
用免疫法复制成大鼠溃疡性结肠炎实验模型,造型动物血清抗大肠抗体均阳性,IgG 显著增高,总 E-玫瑰花结形成率、活性 E-玫瑰花结形成率,淋巴细胞转化率明显降低。结肠组织病理学检查溃疡部位有大量嗜中性白细胞、淋巴细胞、浆细胞、嗜酸性白细胞浸润。从而证实了溃疡性结肠炎的免疫发病学说。经用健脾灵治疗后,实验大鼠结肠粘膜炎症消退、溃疡修复,明显优于补脾益肠丸组和偶氮磺胺吡啶组。其非特异性细胞免疫功能明显提高,血清抗大肠抗体滴度和 IgG 水平显著下降,提示健脾灵治疗溃疡性结肠炎的机理与免疫调节作用有关。
In this paper,the ulcerative colitis model of rats with immuno-method was reported and the mechanism of using Jianpiling for the treatment of ulcerative colitis was studied.The rats of experimental group were subdivided into 3 subgroups(A,B and C),15% Jianpiling suspension was given to rats in subgroup A per os,15% Bupi-Yichang Pill(补脾益肠丸)to rats in subgroup B per os, 4.5% SASP suspension to rats in subgroup C,and same amount of saline to rats in the control group. The therapeutic results showed that the anti-diarrhia,anti-infection and repairing function of tissue by Jianpiling were obviously better than those of Bupi-Yichang Pill and SASP.Serological exams also showed that Jianpiling was much better than other therapeutic groups in increasing nonspecific cellular immunity and lowering level of antibody against large intestine and IgG.These results suggested that the mechanism of Jianpiling on ulcerative colitis was realized by increasing cellular immunity,inhibiting humoral immunity and promoting immune modulation.
