碳酸酐酶Ⅸ和低氧诱导因子-1α在非小细胞肺癌组织中的表达及其临床意义

Expression of Carbonic Anhydrase Ⅸ and Hypoxia-inducible Factor-1α in Non-Small Cell Lung Cancer and Its Significance

【目的】研究碳酸酐酶Ⅸ（CAⅨ）和低氧诱导因子1α（HIF-1α）在非小细胞肺癌（NSCLC）中的表达及意义。【方法】检测80例NSCLC手术标本中的CAⅨ和HIF-1α表达，分析其在癌组织中表达与临床、病理之间的关系。【结果】NSCLC中CAⅨ和HIF1α的表达率分别为71．25％及66．25％，均显著高于正常肺组织（P〈0．01）。CAⅨ在NSCLC的表达与患者临床特征、病理类型无关（P〉0．05），但CAⅨ在淋巴结转移组的阳性表达率明显高于无淋巴结转移组（P〈0．05）；TNM分期Ⅲ～Ⅳ期的cAⅨ阳性表达率高于TNMⅠ～Ⅱ期的CAⅨ阳性表达率（P〈0．05）。而HIF-1α在NSCLC中的表达与患者临床特征、病理类型和临床分期及淋巴结转移均无关（P〉0．05）；CAⅨ和HIF-1α在非小细胞肺癌组织中的表达相关性不强（P〉0．05）。【结论】CAIX参与了NSCLC的发生、发展和侵袭转移，其可能成为抗肿瘤的新靶点。

[Objective] To investigate the expression of carbonic anhydrase Ⅸ（CA Ⅸ ） and hypoxia induc ible factor-1α（HIF-1α） in non-small cell lung cancer（NSCLC） and its significance. [Methods]The expressions of CA ⅨX and HIF-1α in surgical specimens of 80 patients with NSCLC were measured. The relationship of the expressions with clinical features and pathology was analyzed. [Results]The expression of CA Ⅸ and HIF-1α in NSCLC was 71.25% and 66.25 %, respectively, which were significantly higher than those in normal lung tissues（ P 〈0.01）. The expression of CA Ⅸ in NSCLC had no correlation with clinical features and patholog- ical types（ P 〉0. 05）, but the positive expression of CA Ⅸ in lymph node metastasis group was obviously higher than that in non-metastasis group（ P 〈0.05）. The positive expression of CA Ⅸ in stage TⅢ -Ⅳ was higher than that in stage TⅠ-Ⅲ（ P 〈0.05）. The expression of HIF-1αin NSCLC had no correlation with clinical features, pathological types, clinical stage and lymph node metastasis（ P 〉0.05）. The correlation be- tween CA Ⅸ and HIF-1α in NSCLC tissue is not strong（ P〉0.05）. [Conclusion]CA Ⅸ involves in the path- ogenesis, development, invasion and metastases of MSCLC, and may be a new target of antitumor therapy.