摘要
目的探讨鲍曼不动杆菌对氨基糖苷类药物耐药情况和耐药表型及其携带基因的关系。方法收集48株鲍曼不动杆菌,用K-B法和琼脂稀释法检测其对阿米卡星的敏感性,PCR法扩增aac(6′)Ⅰ基因以及3种16SrRNA甲基化酶基因(armA、rmtB及rmtC基因)。结果 72.9%(35/48)菌株对阿米卡星表现为双圈耐药,其中100.0%菌株检出armA基因,未检出rmtB、rmtC基因,77.1%(27/35)菌株检出aac(6′)Ⅰ基因;10.4%(5/48)菌株对阿米卡星普通耐药,均未检出16SrRNA甲基化酶基因,其中80.0%(4/5)菌株检出aac(6′)Ⅰ基因;16.7%(8/48)菌株对阿米卡星敏感,未检出相关耐药基因。结论鲍曼不动杆菌对阿米卡星的耐药情况非常严重;双圈耐药现象与armA基因诱导型表达有关。
Objective To explore the correlation between aminoglycoside resistant situation and phenotype of Acinetobacter baumannii(A.baumannii) and carried genes.Methods 48 strains of A.baumannii were collected and detected for drug susceptibility to Amikacin by disk diffusion method and Mueller-Hinton broth method,and for aac(6’)Ⅰgene and three kinds of 16S rRNA methylase genes(armA,rmtB and rmtC) by polymerases chain reaction(PCR).Results Among 48 strains of A.baumannii,72.9%(35/48) were double-circle resistant to Amikacin,in which the positive rates of armA,rmtB,rmtC and aac(6’)Ⅰ gene were 100.0%(35/35),0.0%(0/35),0/0%(0/35) and 77.1%(27/35),10.4%(5/48)were generally resistant to Amikacin and negative with 16S rRNA methylase genes,in which the positive rate of aac(6’)Ⅰgene was 80.0%(4/5),and 16.7%(8/48) were sensitive to Amikacin and negative with all detected genes related to drug resistance.Conclusion The drug resistance of A.baumannii to Amikacin might be serious.Double-circle resistance possibly be associated with the inducible expression of armA gene.
出处
《国际检验医学杂志》
CAS
2012年第10期1155-1156,共2页
International Journal of Laboratory Medicine