FQ-PCR检测宫颈癌hWAPL基因mRNA及其诊断价值分析

Fluorescent quantitation PCR detection of cervical cancer hWAPL mRNA and its diagnostic value

目的探讨荧光定量聚合酶链反应(FQ-PCR)检测宫颈癌人半翼(hWAPL)基因mRNA的诊断价值。方法使用高危型人乳头瘤病毒(HPV)检测试剂盒以及建立的FQ-PCR检测慢性宫颈炎、宫颈上皮内瘤样变(CIN)和宫颈癌共100例患者hWAPL基因mRNA和高危型HPV。结果宫颈癌组hWAPL基因mRNA表达显著高于慢性宫颈炎组和CINⅠ～Ⅲ组(P<0.05);慢性宫颈炎组与CINⅠ～Ⅲ组比较,差异无统计学意义(P>0.05)。慢性宫颈炎组和CINⅠ～Ⅲ组部分患者中查出高危型HPV,但无宫颈癌变。宫颈癌组高危型HPV检出率显著高于无宫颈癌病变各组。结论高危型HPV检出率与宫颈癌发生密切相关,但hWAPL基因mRNA表达与宫颈癌变更直接相关,更有特异性,有助于宫颈癌的早期诊断。

Objective To explore the diagnostic value of hWAPL mRNA detected by fluorescent quantitation polymerase chain reaction（FQ-PCR）.Methods High-risk type papilloma virus（HPV） test kit and established FQ-PCR technology were performed for the detection of hWAPL mRNA and high-risk HPV in 100 cases of patients with chronic cervicitis,cervical intraepithelial neoplasia（CIN） or cervical cancer.Results The expression level of hWAPL mRNA in cervical cancer group was significantly higher than in chronic cervicitis group and CINⅠ-Ⅲ group（P〈0.05）,but there was no statistical difference between chronic cervicitis group and CINⅠ-Ⅲ group（P〉0.05）.High-risk HPV could be detected in part of patients of chronic cervicitis group and CINⅠ-Ⅲ group,but no cervical lesions could be observed.Detection rate of high-risk HPV in cervical cancer group than in other groups without cervical cancer.Conclusion Detection rate of high-risk HPV might be closely correlated with cervical cancer,but mRNA level of hWAPL gene could be with more closely correlation and specific for cervical cancer,and be more helpful for the early diagnosis of cervical cancer.