人脐血间充质干细胞促进颅脑损伤大鼠修复的研究

Study on human umbilical cord blood MSCs for repair of traumatic brain injury in rats

目的通过建立大鼠颅脑创伤模型,探讨移植人脐血间充质干细胞(MSCs)治疗颅脑损伤的可行性。方法参考Feeney法制作大鼠颅脑损伤模型。实验分移植组、假手术组和对照组。采用盲法在移植后第1、4、12、21天分别对各组实验大鼠进行神经功能评分(NSS)。荧光免疫组化观察移植MSCs21d后神经细胞的特异标志分子神经元核抗原(NeuN)的分布情况。结果成功培养人脐血MSCs。假手术组NSS评分在各时间点均与对照组差异有统计学意义(P<0.01),证明颅脑损伤模型建立成功。实验组大鼠NSS评分在第4、12、21天均显著低于对照组,差异有统计学意义(P<0.05)。荧光免疫组化结果显示,移植MSCs21d后,移植组的神经细胞明显多于对照组。结论在成功建立大鼠颅脑损伤模型的基础上,显示通过移植人脐血MSCs,对大鼠的颅脑损伤有明显的修复促进作用。

Objective To explore the feasibility of human umbilical cord blood mesenchymal stem cells（MSCs） for the treatment of traumatic brain injury in rat model.Methods Traumatic brain injury rat models were constructed according to Feeney method.All rats were divided into transplant group,sham-operated group and control group.Neurological score（NSS） was evaluated in rats of all groups by blind method at 1,4,12 and 21 days after transplantation,respectively.3 weeks after transplantation of MSCs,the distribution of neuron nucleoantigen（NeuN）,specific for nerve cell,was detected by fluorescence immunohistochemistry.Results Human umbilical cord blood MSCs were successfully cultured.NSS scores of sham-operated group,detected at different time points,were significantly different with those of control group（P〈0.01）,which could indicated that the rat model of traumatic brain injury was successfully constructed.NSS scores of transplant group,detected at 4,12 and 21 days after transplantation,were significantly lower than those of control group（P〈0.05）.Results of fluorescence immunohistochemistry demonstrated that,3 weeks after transplantation of MSCs,amount of nerve cells in transplant group was obviously higher than that in control group.Conclusion In vivo experiment in rat model of traumatic brain injury could indicated that transplantation of human cord blood MSCs might be with promoting effects for the repairing of brain injury in rats.