2型糖尿病并发血管疾病男性患者雄激素受体基因(CAG)n多态性研究

The polymorphism of(CAG)n within androgen receptor gene among male patients of type 2 diabetes mellitus complicated by vascular disease

目的研究雄激素受体(AR)基因(CAG)n多态性分布及其与2型糖尿病并发血管疾病的相关性。方法采用序列特异性引物PCR(SSP-PCR)和DNA测序技术检测48例2型糖尿病并发血管疾病的男性患者(A组)的AR基因CAG串联重复序列(CAG-STR)长度,并与60例无血管并发症的2型糖尿病男性患者(B组)和46例健康对照组比较。结果 154例研究对象的CAG重复次数介于13～36次,平均22.2次。A、B、健康对照组CAG重复次数分别为(23.4±3.85)、(22.3±3.57)、(21.9±3.45)次,A组与B组、健康对照组之间的差异均有统计学意义(P<0.05)。CAG重复次数与HDL-C水平呈正相关(r=0.289,P=0.003)。结论 CAG重复序列的长度可能是2型糖尿病并发血管疾病的危险因素,CAG重复序列越长,2型糖尿病患者并发血管疾病的可能性越大。

Objective To study the relationship of androgen receptor（AR） gene（CAG）n polymorphism frequency and angiopathy in male patients of type 2 diabetes mellitus.Methods PCR and DNA sequence methods were used to detect androgen receptor gene CAG short tandem repeat（CAG-STR） repeats’ length in 48 cases of type 2 diabetes patients with vascular complications（group A）,60 type 2 diabetes patients without vascular complications（group B）,and 46 cases of normal controls（normal control group）.Results The numbers of CAG repeats ranged from 13 to 36,with mean value 22.2.The numbers of CAG repeats of group A,group B,and normal control group were 23.4±3.85,22.3±3.57,and 21.9±3.45,respectively.CAG-STR repeats’ length of group A was significantly higher than that of group B and normal control group（P〈0.01）.The numbers of CAG repeats were positively correlated with HDL-C levels.Conclusion Diabetic vascular complications might be associated with androgen receptor CAG gene polymorphism.The number of CAG repeats is likely to be a risk factor of diabetic vascular disease.