IL-23在单纯疱疹病毒Ⅱ型致病中的作用

Role for interleukin-23 in genital infection of herpes simplex virus

目的探讨IL-23在单纯疱疹病毒Ⅱ型(HSV-Ⅱ)致病机制中的作用。方法应用ELISA法检测HSV病毒感染复发患者外周血IL-23、IL-12、IFN-γ、IL-14、IL-10水平,以及细胞培养液中IFN-γ水平,流式细胞仪检测记忆性CD4+T细胞表达IFN-γ的水平,同时设立健康对照组进行比较。结果生殖器疱疹复发患者(RGH)组血清IL-23、IL-12、IFN-γ水平明显低于健康对照组,IL-14、IL-10水平明显高于健康对照组,差异有统计学意义(P<0.01);IL-23剂量依赖性诱导患者单个核细胞(PBMC)产生IFN-γ,并刺激记忆性CD4+T细胞表达IFN-γ。结论 IL-23等Th1型细胞因子在RGH患者体内水平低下,Th1/Th2平衡失调,机体不能有效抑制病毒,是导致病情反复的重要原因;IL-23可能在体内同样刺激T细胞产生IFN-γ,发挥抗病毒效力。

Objective To evaluate the role of IL-23 in genital infection of type Ⅱ herpes simplex virus（HSV-Ⅱ）.Methods Peripheral blood samples from patients with recurrent genital herpes（RGH） were detected by ELISA for interleukin（IL）-23,IL-12,interferon（IFN）-γ,IL-14 and IL-10,and concentration of IFN-γ in cell culture supernatant were also detected.The concentration of IFN-γ in memory CD4＋ T cells were detected by using flow cytometry.Healthy control group was established for comparison.Results Serum levels of IL-23,IL-12,IFN-γ in patients with RGH were significantly lower than in control group,and IL-4 and IL-10 levels were significantly higher（P〈0.01）.Peripheral blood monouclear cells（PBMC） from patients with RGH could produce IFN-γ under IL-23 dose-dependently inducing,which could stimulate memory CD4＋ T cells to express IFN-γ.Conclusion Low level expression of IL-23 and other Th1-type cytokines in RGH patients could restrain effective inhibition of virus in patients by causing Th1/Th2 imbalance,which might be an important reason leading to repeated illness.IL-23 could also stimulate in vivo T cells to produce IFN-γ to play an anti-viral effect.