阴道光滑念珠菌耐药性及氟康唑耐药相关基因的表达

Drug resistance and expression of gene related to fluconazole resistance in Canadida glabrata isolated from vagina

目的研究阴道中光滑念珠菌耐药性及氟康唑耐药相关基因的表达。方法分离、鉴定阴道分泌物中的光滑念珠菌,并进行药敏实验。抽提光滑念珠菌总RNA,通过RT-PCR检测耐药基因CDR1、CDR2和ERG11的相对表达量。结果光滑念珠菌对制霉菌素、两性霉素B的药物敏感性最好,5-氟胞嘧啶次之,对唑类药物的耐药率(41.00%～67.00%)较高;对氟康唑的耐药率为44.00%。RT-PCR结果显示,光滑念珠菌氟康唑耐药组CDR1、CDR2和ERG11基因高表达。结论光滑念珠菌对常用抗真菌药物的耐药率呈上升趋势。光滑念珠菌耐药基因CDR1、CDR2和ERG11的高表达,与临床光滑念珠菌对氟康唑耐药有关。

Objective To explore the drug resistance and expression of fluconazole-resistant genes in Candida glabrata clinical isolates from vagina.Methods Candida glabrata in vaginal secretions were isolated,identified and detected for drug resistance.Total RNA of Candida glabrata isolates were extracted,the expression of CDR1,CDR2 and ERG11 were detected by real-time polymerase chain reaction（PCR）.Results Candida glabrata were more sensitive to nystatin and amphotericine B,followed by 5-fluorocytosine,but resistant to azoles antifungal drugs,with resistant rate from 41.00% to 67.00%.The resistant rate of Candida glabrata clinical isolates to fluconazole was 44.00%.The drug resistance genes CDR1,CDR2 and Erg11 were overexpressed in sluconazole resistant strains.Conclusion The drug resistance of Candida glabrata to commonly used antifungals might be with an ascending trend.CDR1,CDR2 and ERG11 could be overexpressed in drug resistant strains of Candida glabrata,which might be closely correlated with fluconazole resistance.