血清miR-155水平检测在乳腺肿瘤患者早期诊断中的研究

Study on the detection of miR-155 in seum from patients with breast tumor

目的探讨血清miR-155检测在乳腺肿瘤早期诊断中的作用。方法提取20例早、中期乳腺肿瘤患者以及10例健康人血清中的总RNA,利用荧光定量技术系统分析与乳腺肿瘤极其相关的miR-155在血清中的表达情况。结果 miR-155在乳腺肿瘤患者血清中的表达与健康人相比均呈现出显著的高表达,两者表达水平经Mann-Whitney检验,P<0.05,说明miR-155的表达水平在乳腺肿瘤患者与健康人之间存在明显差异。结论疾病特异性miRNA或可作为肿瘤早期检测的血清学分子标志物。

Objective To study the significance of the detection of miR-155 in serum for the early diagnosis of breast tumor.Methods Total RNA in serum were extracted in 20 patients with newly diagnosed breast tumor and 10 healthy subjects.Real-time polymerase chain reaction（PCR） was used to detect the expression of miR-155 in serum samples.Results Expression level of miR-155 in patients with breast tumor was significantly higher than in healthy controls（P〈0.05）.Conclusion miRNA specific for diseases could be used as serologial biomarkers for early diagnosis of cancer.